| Grant number: | 14/17967-4 |
| Support Opportunities: | Regular Research Grants |
| Start date: | December 01, 2014 |
| End date: | November 30, 2016 |
| Field of knowledge: | Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture |
| Principal Investigator: | Maria José Tavares Ranzani de Paiva |
| Grantee: | Maria José Tavares Ranzani de Paiva |
| Host Institution: | Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil |
| Associated researchers: | Danielle de Carla Dias ; Fábio Rosa Sussel ; Giovani Sampaio Gonçalves ; Leonardo Tachibana |
Abstract
The project will be conducted with Nile tilapia, Oreochromis niloticus, in APTA/Pólo Centro Oeste/UPD Pirassununga/SP, and will feature two experiments with diets supplemented with probiotics PAS - TR® 4 (Bacillus cereus 0x108 CFU g-1 and Bacillus subtilis 4.0 x108 CFU g-1) and DBA® (Lactobacillus acidophilus - 3.5 g x109UFC-1, Enterococcus faecium - 3.5 g x109UFC-1 and Bifidobacteria - x109UFC 3.5 g-1) and prebiotics Actigen (MOS), chitosan and macroalgae Kappaphycus alvarezii tested separately and in symbiosis (probiotic and prebiotic). In both experiments, the first step to evaluate the growth performance, hematological and immunological parameters, body composition of the fish, gene expression, recovery of intestinal microbiota and intestinal histology of Nile tilapia fed with specific diets for each treatment (control, probiotic, prebiotic 1 and and symbiotic 1 and 2) will be performed, during 63 days. The fish will be distributed in a completely randomized design with six treatments and four replications for each experiment. In the second stage, 30 fish per treatment will be experimentally infected " in vivo " with Aeromonas hydrophila after 21 days of feeding the respective treatments. Within 15 days post -infection the relative protection index and clinical signs of infection with A. hydrophila be observed. The experimental design of this stage will be completely randomized design with 6 treatments and 3 replications in each experiment. The results of both phases will be analyzed by SAS ® program, by analysis of variance (ANOVA) followed by Tukey test at 5 % significance . (AU)
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