Qualitative analysis of delayed graft viability of autogenous adipose tissue in minipigs

• Grant number: 14/19463-3
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2015
• End date: July 31, 2016
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Alexandre Mendonça Munhoz
• Grantee: Alexandre Mendonça Munhoz
• Host Institution: Hospital Sírio-Libanês. Sociedade Beneficente de Senhoras (SBSHSL). São Paulo , SP, Brazil
• Associated researchers: Eduardo Gustavo Pires de Arruda ; Walter Koiti Matsumoto

Abstract

Skin graft or lipograft has always been considered a very high potential technique for Plastic Surgery, particularly for post-surgical oncologic Breast Reconstruction. Increased research and wider use of stem cell therapies, along with the finding of adipose tissue potential as the source for those cells motivated additional interest in lipograft protocols.However, the unpredictable clinical course of fat grafting as well as the lack of technical standards in literature have always hindered wider use and improvement of that procedure. The purpose of this controlled, prospective study is to use an experimental animal model (Minipigs) that is viable and reproducible in order to evaluate the role played by adipose tissue collection (liposuction cannulas and en bloc resection using a punch circular slide); the diameter of the instrument used for tissue collection (0,3 and 0,6 mm); and tissue pressure in the feasibility of autogenous adipose tissue grafting. The samples will be submitted to adipose tissue grafting at the Teaching and Research Institute, Sirio-Libanês Hospital in São Paulo (IEP-Sírio-Libanês). General anesthesia and aseptic technique will be used. Following collection techniques, the grafts will be divided into eight groups, defined by the instrument used and the presence or the absence of high tissue pressure: 3-mm diameter liposuction cannula (with and without tissue pressure increase); 6-mm cannula (with and without pressure increase), 3-mm punch (with and without pressure increase); and 6-mm punch (with and without pressure increase). The grafts will be placed intramuscularly in hind legs of samples. Hind legs were chosen due to good vascularization and sufficiently extensive area to receive many grafts. In the post-surgery period, the samples will receive analgesia and antibiotic prophylaxis. After four weeks, the samples will be reanesthetized for excisional biopsy of grafted adipose tissue. To compare tissue feasibility of tissue grafts in the four groups mentioned earlier, the material will be submitted to qualitative analysis for the following parameters: feasible adipocytes, necrosis, fibrosis, and blood vessels. The comparison will be performed through histopathological study with hematoxylin and eosin staining as well as immunohistochemical study through the identification of TNF-alpha (cytokine in one of the apoptotic pathways in adipose tissue) with monoclonal antibodies in adipose tissue grafts. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIRES DE ARRUDA, EDUARDO GUSTAVO; MUNHOZ, ALEXANDRE MENDONCA; MATSUMOTO, WALTER; UEDA, THIAGO; COUDRY, RENATA DE ALMEIDA; GEMPERLI, ROLF. Qualitative analysis of the viability of autogenous fat grafts grafted in different environments of interstitial pressure. Preliminary results and description of a new experimental model in mini-pigs. Acta Cirurgica Brasileira, v. 32, n. 11, p. 891-902, NOV 2017. (14/19463-3)

PIRES DE ARRUDA, EDUARDO GUSTAVO; MUNHOZ, ALEXANDRE MENDONCA; MATSUMOTO, WALTER; UEDA, THIAGO; MONTAG, EDUARDO; OKADA, ALBERTO; COUDRY, RENATA DE ALMEIDA; DE CASTRO, ISAC; GEMPERLI, ROLF. Impact of Fat Graft Thickness and Harvesting Technique on Adipocyte Viability in a New Porcine Experimental Model: An Immunohistochemical Analysis. AESTHETIC SURGERY JOURNAL, v. 41, n. 6, p. NP616-NP630, JUN 2021. (14/19463-3)

PIRES DE ARRUDA, EDUARDO GUSTAVO; MUNHOZ, ALEXANDRE MENDONCA; MATSUMOTO, WALTER; UEDA, THIAGO; COUDRY, RENATA DE ALMEIDA; GEMPERLI, ROLF. Qualitative analysis of the viability of autogenous fat grafts grafted in different environments of interstitial pressure. Preliminary results and description of a new experimental model in mini-pigs. Acta Cirurgica Brasileira, v. 32, n. 11, p. 12-pg., 2017-11-01. (14/19463-3)