Effect of vitamin 25 (OH) d on the expression of Toll-like receptor and inflammatory mediators in the uremic environment

• Grant number: 14/14192-1
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: May 31, 2017
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Maria Aparecida Dalboni
• Grantee: Maria Aparecida Dalboni
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

In addition to the vitamin D being associated with the metabolism of calcium and phosphorus, it has been described that vitamin D is also involved in the modulation of cell mechanisms of innate immunity. The discovery that the vitamin D receptor (VDR) and 1±-hydroxylase enzyme (CYP27B1) are present in cells of the immune system has revolutionized the field of immunology associated with vitamin D. It has been reported that patients with chronic kidney disease (CKD) have a deficiency of this vitamin due to loss of renal mass. Furthermore, these patients have episodes of major infection and inflammation compared to the normal population. Studies have shown that vitamin D is capable of the regulate the expression of endogenous antimicrobial peptides against specific infectious agents and to regulate the expression of toll-like receptors present on neutrophils, monocytes and lymphocytes; and once activated, result in greater synthesis of inflammatory mediators.Thus, it is possible that a deficiency of vitamin D found in patients with CKD is one of the mechanism that results in a reduced response to infectious insults, and increased incidence of inflammation in this population. In this study, we will use an "in vitro" model to evaluate the effect of vitamin D on the expression of toll-like receptors and inflammatory mediators' modulation against insult uremic serum.Thus, studying the effect of vitamin D on the modulation of inflammatory response in a model of cells of innate immunity against an uremic environment concerns as a future therapeutic strategy of low cost and will assess mechanisms involved in this modulation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REBELLO, JACQUELINE FERRITTO; OLIVEIRA BRITO, RODRIGO BARBOSA; DOS SANTOS OLIVEIRA, WILLIAN RUBENS; RIBEIRO, GABRIEL FELIX; GRABULOSA, CAREN CRISTINA; DIAS, ALANA SANTOS; DELLE, HUMBERTO; DALBONI, MARIA APARECIDA. Effect of uremia on downregulation of TLR4 and upregulation of cytokines in endothelial cell. FASEB JOURNAL, v. 31, p. 2-pg., 2017-04-01. (14/14192-1)

CARDOSO RODRIGUES, GABRIELA GOMES; DELLE, HUMBERTO; OLIVEIRA BRITO, RODRIGO BARBOSA; CARDOSO, VINICIUS OLIVEIRA; SANTOS FERNANDES, KRISTIANNE PORTA; MESQUITA-FERRARI, RAQUEL AGNELLI; CUNHA, REGIANE STAFIM; MARQUES STINGHEN, ANDREA EMILIA; DALBONI, MARIA APARECIDA; BARRETO, FELLYPE CARVALHO. Indoxyl Sulfate Contributes to Uremic Sarcopenia by Inducing Apoptosis in Myoblasts. ARCHIVES OF MEDICAL RESEARCH, v. 51, n. 1, p. 21-29, JAN 2020. (14/14192-1)

DE OLIVEIRA BRITO, RODRIGO BARBOSA; REBELLO, JACQUELINE FERRITTO; DOS SANTOS OLIVEIRA, WILLIAN RUBENS; GRABULOSA, CAREN CRISTINA; TELES MATOS, YVES SIVA; MALTA, CAMILA SOARES; DELLE, HUMBERTO; DALBONI, MARIA APARECIDA. 25 (OH) D3 treatment improves inflammatory pathway on monocytes lineage (U937) in uremic environment. FASEB JOURNAL, v. 31, p. 1-pg., 2017-04-01. (14/14192-1)