Dissecting essential roles of chicken interferon stimulated genes in the pathobiology of poultry viruses

• Grant number: 14/50696-4
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: August 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Agreement: BBSRC, UKRI
• Principal Investigator: Clarice Weis Arns
• Grantee: Clarice Weis Arns
• Principal researcher abroad: Muhammad Munir
• Institution abroad: Pirbright Institute, England
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

To initiate successful virus replication cycle, viruses have to breach a cascade of host cellular responses. Among these barriers, innate immune responses are most potent and independent to nature of pathogens. The objectives of this work are to define the interactions of chicken interferon stimulated genes (chISGs) with diverse poultry viruses that are of economic importance both in Brazil and UK. Our preliminary transcriptomic and expression analysis indicate that chlSGs are the most potent responses of the chicken infected with Marek's disease virus (MOV) indicating that chlSGs essentially mediate pathobiology of viruses. In this proposal, we will dissect the significance and breadths of chlSGs against poultry viruses using large-scale, genome-wide and high through put screen platforms. Specifically, we will simultaneously measure the temporal expression of ISGs that synergistically or antagonistically regulate specific virus replication using a pre-established lentivirus-based ISG library. We will combine this data with results gain through shRNA mediated silencing screening to catalogue chlSGs with broad-spectrum or virus specific chlSGs. These data will be integrated within mechanistic studies to provide an incredible rich data on how viruses interact with host immune responses, essentially contributed by ISGs. and the ways viruses have adapted to circumvent these responses. These experimentations will be performed with avian influenza virus, avian paramyxoviruses and Marek's disease virus in The Pirbright Institute, UK and with avian metapneumoviruses and infectious bursal disease virus in State University of Campinas, Brazil using homogeneous system in two laboratory settings. Finally, the gained information will be utilized to establish a cell line having profound favorable effects on the growth kinetics. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIZOTTO, LAIS S.; SCAGION, GUILHERME P.; CARDOSO, TEREZA C.; SIMAO, RAPHAEL M.; CASERTA, LEONARDO C.; BENASSI, JULIA C.; KEID, LARA B.; OLIVEIRA, TRICIA M. F. DE S.; SOARES, RODRIGO M.; ARNS, CLARICE W.; et al. Complete Genome Sequence of an Avian Metapneumovirus Subtype A Strain Isolated from Chicken (Gallus gallus) in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 5, n. 29, JUL 2017. (14/50696-4, 15/11510-5)

SANTHAKUMAR, DIWAKAR; ROHAIM, MOHAMMED ABDEL MOHSEN SHAHAAT; HUSSEIN, HUSSEIN A.; HAWES, PIPPA; FERREIRA, HELENA LAGE; BEHBOUDI, SHAHRIAR; IQBAL, MUNIR; NAIR, VENUGOPAL; ARNS, CLARICE W.; MUNIR, MUHAMMAD. Chicken Interferon-induced Protein with Tetratricopeptide Repeats 5 Antagonizes Replication of RNA Viruses. SCIENTIFIC REPORTS, v. 8, MAY 1 2018. (14/50696-4)

MARTINI, MATHEUS C.; CASERTA, LEONARDO C.; DOS SANTOS, MARCIA M. A. B.; BARNABE, ANA C. S.; DURAES-CARVALHO, RICARDO; PADILLA, MARINA A.; SIMAO, RAPHAEL M.; RIZOTTO, LAIS S.; SIMAS, PAULO V. M.; BASTOS, JULIANA C. S.; et al. Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks. AVIAN PATHOLOGY, v. 47, n. 3, p. 286-293, 2018. (14/50696-4, 15/11510-5)

RIZOTTO, LAIS S.; SCAGION, GUILHERME P.; CARDOSO, TEREZA C.; SIMAO, RAPHAEL M.; CASERTA, LEONARDO C.; BENASSI, JULIA C.; KEID, LARA B.; OLIVEIRA, TRICIA M. F. DE S.; SOARES, RODRIGO M.; ARNS, CLARICE W.; et al. Complete Genome Sequence of an Avian Metapneumovirus Subtype A Strain Isolated from Chicken (Gallus gallus) in Brazil. GENOME ANNOUNCEMENTS, v. 5, n. 29, p. 2-pg., 2017-07-01. (15/11510-5, 14/50696-4)