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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Avian coronavirus isolated from a pigeon sample induced clinical disease, tracheal ciliostasis, and a high humoral response in day-old chicks

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Author(s):
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Martini, Matheus C. [1] ; Caserta, Leonardo C. [1] ; dos Santos, Marcia M. A. B. [2] ; Barnabe, Ana C. S. [1] ; Duraes-Carvalho, Ricardo [1] ; Padilla, Marina A. [1] ; Simao, Raphael M. [3] ; Rizotto, Lais S. [3] ; Simas, Paulo V. M. [1] ; Bastos, Juliana C. S. [1] ; Cardoso, Tereza C. [4] ; Felippe, Paulo A. N. [1] ; Ferreira, Helena L. [3, 5] ; Arns, Clarice W. [1]
Total Authors: 14
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Lab Anim Virol, Campinas, SP - Brazil
[2] Univ Fed Juiz de Fora, ICB, Juiz De Fora, MG - Brazil
[3] Univ Sao Paulo, FMVZ, Fac Vet Med & Anim Sci, Postgrad Program Expt Epidemiol Zoonoses, Sao Paulo, SP - Brazil
[4] Univ Estadual Paulista, Coll Vet Med, Lab Anim Virol & Cell Culture, DAPSA Dept, Aracatuba, SP - Brazil
[5] Univ Sao Paulo, FZEA, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: AVIAN PATHOLOGY; v. 47, n. 3, p. 286-293, 2018.
Web of Science Citations: 0
Abstract

The detection of avian coronaviruses (AvCoV) in wild birds and the emergence of new AvCoV have increased in the past few years. In the present study, the pathogenicity of three AvCoV isolates was investigated in day-old chicks. One AvCoV isolated from a pigeon, which clustered with the Massachusetts vaccine serotype, and two AvCoV isolated from chickens, which grouped with a Brazilian genotype lineage, were used. Clinical signs, gross lesions, histopathological changes, ciliary activity, viral RNA detection, and serology were evaluated during 42 days post infection. All AvCoV isolates induced clinical signs, gross lesions in the trachea, moderate histopathological changes in the respiratory tract, and mild changes in other tissues. AvCoV isolated from the pigeon sample caused complete tracheal ciliostasis over a longer time span. Specific viral RNA was detected in all tissues, but the highest RNA loads were detected in the digestive tract (cloacal swabs and ileum). The highest antibody levels were also detected in the group infected with an isolate from the pigeon. These results confirm the pathogenicity of Brazilian variants, which can cause disease and induce gross lesions and histopathological changes in chickens. Our results suggest that non-Galliformes birds can also play a role in the ecology of AvCoV. (AU)

FAPESP's process: 14/50696-4 - Dissecting essential roles of chicken interferon stimulated genes in the pathobiology of poultry viruses
Grantee:Clarice Weis Arns
Support type: Regular Research Grants
FAPESP's process: 15/11510-5 - Disclosing the host-pathogen and Pathogen-Pathogen interactions using metagenomics and high throughput sequencing approaches
Grantee:Helena Lage Ferreira
Support type: Regular Research Grants