Study of cells and tissues involved in the process of repair, and revitalization / revascularization of dental pulp

• Grant number: 14/18623-7
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: February 28, 2017
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Alexandre Augusto Zaia
• Grantee: Alexandre Augusto Zaia
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Many injuries may assail the tooth lifespan as caries, attrition, abrasion, erosion, trauma, dental restorative procedures, and materials resulting in partial or total loss of the connective pulp tissue. The tissue engineering has been used in an attempt to restore the pulp through pulp repair techniques, when there is partial loss of pulp tissue or by using revitalization/revascularization techniques, in cases of total loss of pulp tissue in immature teeth with incomplete apical closure. The mechanism of repair or revitalization/revascularization that follows after an injury to dental pulp is important for the survival of the tooth and involves a series of biological processes that need to be fully elucidated. Little is known about the molecular mechanism involved in the process of dental healing. Most precisely, the origin and the process of recruitment and differentiation of cells involved in dental pulpal repair or dental pulp revitalization/revascularization, in addition the behavior of these cells to the different materials and chemicals used in these techniques. In view of the above is objective of this research project: 1) To analyze the participation of non-residents hematopoietic stem cells (HSC) in the pulp repair after pulp exposure and capping using a transgenic GFP (green fluorescent protein) animals model, 2) To Identify whether different capping materials of dental pulp have the potential to cause painful sensations through the expression of substance P and NK receptors in cultures of immortalized fibroblasts (3T3) and 3) To investigate whether the chemicals used during decontamination of the root canal in the dental pulp revitalization/revascularization technique may interfere with adhesion of undifferentiated mesenchymal cells to dentin compromising treatment success. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FROZONI, MARCOS; MARQUES, MARCELO ROCHA; HAMASAKI, SILVIA KAORU; MOHARA, NELSON TSUTOMU; SOARES, ADRIANA DE JESUS; ZAIA, ALEXANDRE AUGUSTO. Contribution of Bone Marrow-derived Cells to Reparative Dentinogenesis Using Bone Marrow Transplantation Model. JOURNAL OF ENDODONTICS, v. 46, n. 3, p. 404-412, MAR 2020. (14/18623-7, 13/50251-0)