First molecular characterization of Cryptosporidium spp. infecting buffalo claves in Brazil

• Grant number: 15/50049-1
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: May 01, 2015
• End date: October 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Katia Denise Saraiva Bresciani
• Grantee: Katia Denise Saraiva Bresciani
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Whit the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged to six months. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S RNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as five species C. parvum, was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes. (AU)