Molecular and biochemical characterization of thyroid carcinomas for clinical diagnosis

• Grant number: 13/14464-9
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: August 31, 2017
• Field of knowledge: Engineering - Biomedical Engineering
• Principal Investigator: Renata de Azevedo Canevari
• Grantee: Renata de Azevedo Canevari
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated researchers: Airton Abrahao Martin

Abstract

In Brazil, the INCA estimates for the year 2014 a total of 9.200 new cases of thyroid cancer, with an estimated risk of 30 cases per 100,000 women. Also according to the INCA during the last decades in most countries the incidence rates of this type of tumor showed a pattern of slow growth, but continuous (about 1% per year). The commonly used diagnostic thyroid cancer occurs from clinical perception and association of fine needle aspiration and ultrasonography, which have a high number of inconclusive results for several pathologies, clearly demonstrating the need to search for new techniques and technologies diagnosis. Within this context, the study of genetic and molecular profile of thyroid lesions has improved the knowledge of the tumor biology and can be integrated into routine diagnostic in order to benefit the pathological analysis and clinical practice and so lead to advances in treatment of patients with these tumors. In addition, Raman spectroscopy presents as a powerful technique for identification and characterization of thyroid lesions. The reason for this is the fact that its ability to provide molecular information and then allow the perception of early biochemical changes before morphological changes. Considering these aspects, this project aims to study the molecular and biochemical differences of the thyroid gland to normal and tumor tissues, by molecular biology analysis (qRT-PCR) and confocal Raman spectroscopy, respectively, to increase sensitivity and specificity of diagnosis of this carcinoma. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, RAISSA MONTEIRO; PUPIN, BRENO; BHATTACHARJEE, TANMOY TAPOBRATA; VAMONDES KULCSAR, MARCO AURELIO; UNO, MIYUKI; CHAMMAS, ROGER; CANEVARI, RENATA DE AZEVEDO. ATR-FTIR spectroscopy and CDKN1C gene expression in the prediction of lymph nodes metastases in papillary thyroid carcinoma. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 228, MAR 5 2020. (13/14464-9, 09/18440-1)

PEREIRA VIANA, MAGDA ROGERIA; ALVES MELO, IGOR MARTINS; PUPIN, BRENO; RANIERO, LEANDRO JOSE; CANEVARI, RENATA DE AZEVEDO. Molecular detection of HPV and FT-IR spectroscopy analysis in women with normal cervical cytology. Photodiagnosis and Photodynamic Therapy, v. 29, MAR 2020. (13/14464-9)

RICCI, R.; LEITE, N. C. S.; DA-SILVA, N. S.; PACHECO-SOARES, C.; CANEVARI, R. A.; MARCIANO, F. R.; WEBSTER, T. J.; LOBO, A. O.. Graphene oxide nanoribbons as nanomaterial for bone regeneration: Effects on cytotoxicity, gene expression and bactericidal effect. Materials Science & Engineering C-Materials for Biological Applications, v. 78, p. 341-348, SEP 1 2017. (11/17877-7, 11/20345-7, 13/14464-9, 13/20054-8, 15/09697-0, 09/18440-1, 16/00575-1)

DA SILVA, RAISSA MONTEIRO; SANTOS, JOYCE NASCIMENTO; UNO, MIYUKI; CHAMMAS, ROGER; VAMONDES KULCSAR, MARCO AURELIO; SANT'ANNA, LUCIANA BARROS; CANEVARI, RENATA DE AZEVEDO. CCNA1 gene as a potential diagnostic marker in papillary thyroid cancer. ACTA HISTOCHEMICA, v. 122, n. 8, DEC 2020. (09/18440-1, 13/14464-9)

RYMSZA, TACIANA; RIBEIRO, ELIANE ALINE; DAS CHAGAS E SILVA DE CARVALHO, LUIS FELIPE; BHATTACHARJEE, TANMOY; CANEVARI, RENATA DE AZEVEDO. Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 196, p. 238-246, MAY 5 2018. (13/14464-9, 09/18440-1, 14/05978-1)

ALVES MELO, IGOR MARTINS; PEREIRA VIANA, MAGDA ROGERIA; PUPIN, BRENO; BHATTACHARJEE, TANMOY TAPOBRATA; CANEVARI, RENATA DE AZEVEDO. PCR-RFLP and FTIR-based detection of high-risk human papilloma virus for cervical cancer screening and prevention. BIOCHEMISTRY AND BIOPHYSICS REPORTS, v. 26, JUL 2021. (13/14464-9)

SILVA SOARES, LUIS EDUARDO; MAGALHAES, JUAREZ DA SILVA; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. Surface characteristics of a modified acidulated phosphate fluoride gel with nano-hydroxyapatite coating applied on bovine enamel subjected to an erosive environment. MICROSCOPY RESEARCH AND TECHNIQUE, v. 81, n. 12, p. 1456-1466, DEC 2018. (11/17877-7, 11/20345-7, 13/14464-9, 07/07984-5, 09/18440-1)

QUEIROZ, JOAO PAULO DA SILVA; PUPIN, BRENO; BHATTACHARJEE, TANMOY TAPOBRATA; UNO, MIYUKI; CHAMMAS, ROGER; KULCSAR, MARCO AURELIO VAMONDES; CANEVARI, RENATA DE AZEVEDO. Expression data of FOS and JUN genes and FTIR spectra provide diagnosis of thyroid carcinoma. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 304, p. 8-pg., 2024-01-05. (19/10211-5, 09/18440-1, 13/14464-9)