Root apoplastic and symplastic 111Cd-uptake, oxidative stress and ultra-structural changes in Panicum maximum plants supplied with sulfur

• Grant number: 14/18735-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2015
• End date: May 31, 2017
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: José Lavres Junior
• Grantee: José Lavres Junior
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Adriana Pinheiro Martinelli

Abstract

Cadmium (Cd) difficult the development of plants, whereupon can be minimized by sulfur (S) supply to plants grown in contaminated environments and the use of tolerant genotypes with high mass production for phytoremediation. The main objective is to evaluate the effect of S on the Cd uptake and its distribution on plant tissue, evaluate the ultra-structural changes on the root and leaves tissues, assay the oxidative stress and the photosynthetic parameters [CO2 assimilation (PN), stomatal conductance (gs), transpiration (E), intercellular CO2 concentration (pI), chlorophyll fluorescence (the effective quantum efficiency of photosystem II and apparent electron transport)] driven by Cd (111Cd) uptake, as well as to determine Cd content on the apoplast and symplast of Panicum maximum cv. Tanzania. The hypothesis to be verified is that well S-nourished plants increase the synthesis of glutathione and phytochelatins, attenuating oxidative stress caused by Cd excess. It is also expected that the chlorophyll concentration and the effective quantum of photosystem II increase with the S supply due to the attenuation of oxidative stress and lower Cd transport from roots to shoots due to higher Cd apoplast uptake. Higher Cd apoplastic accumulation (than symplastic) has been described as an effective mechanism of heavy metal tolerance. Therefore, it is expected that the appropriate S supply to Panicum maximum cv. Tanzania may contribute to the advancement of phytoremediation in environments with low to moderate Cd contamination. The experiment will be carried out in a greenhouse, using nutrient solution with three S rates (0.1, 1.9 and 3.7 mmol L-1) and three Cd rates (0.0, 0.1 and 0.5 mmol L-1), in a factorial 3x3 experimental arrange. These following evaluations will be held at the end of the plant growth: morphological, productive, nutritional parameters, Cd-apoplast and symplast content, ultra-structural changes, soluble protein content, H2O2 content, lipid peroxidation, reduced glutathione, oxidized glutathione, the total glutathione, activity of superoxide dismutase, ascorbate peroxidase, catalase, guaiacol peroxidase and glutathione reductase, PN, gs, E, pI, minimal and maximal chlorophyll fluorescence, fluorescence in dynamic and maximal equilibrium state. The results will be submitted to analysis of variance and the treatment means are compared by Tukey test at 5% significance. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVEIRA RABELO, FLAVIO HENRIQUE; BORGO, LUCELIA; KEUNEN, ELS; DOS REISE, ANDRE RODRIGUES; MARTINELLI, ADRIANA PINHEIRO; CUYPERS, ANN; LAVRES, JOSE. Enzymatic antioxidants-Relevant or not to protect the photosynthetic system against cadmium-induced stress in Massai grass supplied with sulfur?. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 155, p. 702-717, NOV 2018. (14/18735-0, 17/12367-7, 14/16731-7, 15/21562-2)

RABELO, FLAVIO HENRIQUE SILVEIRA; VANGRONSVELD, JACO; BAKER, ALAN J. M.; VAN DER ENT, ANTONY; ALLEONI, LUIS REYNALDO FERRACCIU. Are Grasses Really Useful for the Phytoremediation of Potentially Toxic Trace Elements? A Review. FRONTIERS IN PLANT SCIENCE, v. 12, NOV 24 2021. (14/18735-0, 12/11859-0, 15/21562-2, 18/07190-3, 17/11299-8, 12/12894-3, 14/16731-7)

RABELO, FLAVIO HENRIQUE SILVEIRA; LAVRES, JOSE; RAMIRES, THIAGO GENTIL; ALLEONI, LUIS REYNALDO FERRACCIU. Diagnosis and recommendation integrated system and nutritional balance index reveal Cd-induced nutritional disorders in Panicum maximum assayed for Cd phytoextraction. BIOREMEDIATION JOURNAL, v. 24, n. 4, p. 265-282, NOV 5 2020. (14/18735-0, 15/21562-2, 12/12894-3, 17/11299-8, 14/16731-7, 12/11859-0, 18/07190-3)

SILVEIRA RABELO, FLAVIO HENRIQUE; MORAL, RAFAEL DE ANDRADE; LAVRES, JOSE. Integrating Biochemical, Morpho-physiological, Nutritional, and Productive Responses to Cd Accumulation in Massai Grass Employed in Phytoremediation. WATER AIR AND SOIL POLLUTION, v. 230, n. 5, MAY 2019. (15/21562-2, 14/18735-0, 14/16731-7, 14/03310-3)

SILVEIRA RABELO, FLAVIO HENRIQUE; JORDAO, LUIZ TADEU; LAVRES, JOSE. A glimpse into the symplastic and apoplastic Cd uptake by Massai grass modulated by sulfur nutrition: Plants well-nourished with S as a strategy for phytoextraction. Plant Physiology and Biochemistry, v. 121, p. 48-57, DEC 2017. (15/21562-2, 14/18735-0, 14/16731-7)

SILVEIRA RABELO, FLAVIO HENRIQUE; LUX, ALEXANDER; ROSSI, MONICA LANZONI; MARTINELL, ADRIANA PINHEIRO; CUYPERS, ANN; LAVRES, JOSE. Adequate S supply reduces the damage of high Cd exposure in roots and increases N, S and Mn uptake by Massai grass grown in hydroponics. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 148, p. 35-46, APR 2018. (15/21562-2, 14/18735-0, 14/16731-7)

SILVEIRA RABELO, FLAVIO HENRIQUE; FERNIE, ALISDAIR ROBERT; NAVAZAS, ALEJANDRO; BORGO, LUCELIA; KEUNEN, ELS; DE ALCANTARA DA SILVA, BERENICE KUSSUMOTO; CUYPERS, ANN; LAVRES, JOSE. A glimpse into the effect of sulfur supply on metabolite profiling, glutathione and phytochelatins in Panicum maximum cv. Massai exposed to cadmium. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 151, p. 76-88, JUL 2018. (15/21562-2, 14/18735-0, 14/16731-7)