Relationship between sulfur and cadmium (111Cd) detoxification mechanisms in Panicum maximum cv. Tanzania

• Grant number: 14/16731-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2015
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: José Lavres Junior
• Grantee: Flávio Henrique Silveira Rabêlo
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 15/21562-2 - Synthesis of phytochelatins and expression of genes involved in the control of oxidative stress and in the cadmium detoxification in Panicum maximum Jacq. cv. Tanzania supplied with sulfur, BE.EP.DR

Abstract

Depending on the amount absorbed and location in plant tissues, cadmium (Cd) might hamperthe development and growth of the plants with greater or lesser intensity, making difficult the cultivation of these plants in contaminated environments. This problem stimulates the search for alternatives that minimize the impacts caused by Cd toxicity on the environment. The sulfur supply (S) for plants grown in contaminated environments is an option to minimize the intensity of damage caused by Cd to plants. This technology can also be promising to increase the phytoextraction of the toxic element, especially to plants that have a high biomass yield potential. So the main aim with this study is to verify the main Cd detoxification mechanisms of Panicum maximum cv. Tanzania and its potential for phytoextraction, as well as to analyze the effect of S supply on: Cd uptake and itsdistribution on plant tissues; location of Cd in root system; evaluate the biosynthesis of organic acids; biosynthesis of cysteine, glutathione, phytochelatins, and other thiol compounds; and analyze the ultra-structural changes on roots and shoots tissues (stem and leaf blades). The hypothesis to bechecked is the S supply increase the biosynthesis of cysteine, glutathione, phytochelatins, and other thiol compounds associated with other complexing Cd compounds, such as organic acids, that might decrease the damage caused by Cd toxicity. Thus, it is expected that the ultra-structural changes(notably on the chloroplasts) shown on sulfur-well nourished plants (Panicum maximum cv. Tanzania) are less pronounced than S-deficient plants. It is also expected that the Panicum maximum cv. Tanzania accumulate more Cd on root apoplast, since this fact is crucial for tolerance to heavy metals in dicot plants. In general, it is expected that the tolerance of well S-nourished Panicum maximum cv. Tanzania to Cd fulfill the S fertilization recommendation in environments contaminated by Cd. The experiment will be carried out in a greenhouse in nutrient solution, with three rates of S (0.1, 1.9 and 3.7 mmol L-1) and three rates of Cd (0.0, 0.1 and 0.5 mmol L-1) in a 3x3 factorial scheme. At the end of the growth period of the plants, the follow assessments will be evaluated: dry matter yield, the concentrations and accumulations of Cd and S, as well as Cd concentration on the root apoplast and symplast, Cd localization to the root system, ultra-structural changes, concentration of organic acids and biosynthesis cysteine, glutathione, phytochelatins, and other thiol compounds. The results will besubmitted to analysis of variance and treatment means are compared by Tukey test at 5% significance level. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVEIRA RABELO, FLAVIO HENRIQUE; BORGO, LUCELIA; KEUNEN, ELS; DOS REISE, ANDRE RODRIGUES; MARTINELLI, ADRIANA PINHEIRO; CUYPERS, ANN; LAVRES, JOSE. Enzymatic antioxidants-Relevant or not to protect the photosynthetic system against cadmium-induced stress in Massai grass supplied with sulfur?. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 155, p. 702-717, NOV 2018. (14/18735-0, 17/12367-7, 14/16731-7, 15/21562-2)

RABELO, FLAVIO HENRIQUE SILVEIRA; VANGRONSVELD, JACO; BAKER, ALAN J. M.; VAN DER ENT, ANTONY; ALLEONI, LUIS REYNALDO FERRACCIU. Are Grasses Really Useful for the Phytoremediation of Potentially Toxic Trace Elements? A Review. FRONTIERS IN PLANT SCIENCE, v. 12, NOV 24 2021. (14/18735-0, 12/11859-0, 15/21562-2, 18/07190-3, 17/11299-8, 12/12894-3, 14/16731-7)

RABELO, FLAVIO HENRIQUE SILVEIRA; LAVRES, JOSE; RAMIRES, THIAGO GENTIL; ALLEONI, LUIS REYNALDO FERRACCIU. Diagnosis and recommendation integrated system and nutritional balance index reveal Cd-induced nutritional disorders in Panicum maximum assayed for Cd phytoextraction. BIOREMEDIATION JOURNAL, v. 24, n. 4, p. 265-282, NOV 5 2020. (14/18735-0, 15/21562-2, 12/12894-3, 17/11299-8, 14/16731-7, 12/11859-0, 18/07190-3)

SILVEIRA RABELO, FLAVIO HENRIQUE; MORAL, RAFAEL DE ANDRADE; LAVRES, JOSE. Integrating Biochemical, Morpho-physiological, Nutritional, and Productive Responses to Cd Accumulation in Massai Grass Employed in Phytoremediation. WATER AIR AND SOIL POLLUTION, v. 230, n. 5, MAY 2019. (15/21562-2, 14/18735-0, 14/16731-7, 14/03310-3)

SILVEIRA RABELO, FLAVIO HENRIQUE; JORDAO, LUIZ TADEU; LAVRES, JOSE. A glimpse into the symplastic and apoplastic Cd uptake by Massai grass modulated by sulfur nutrition: Plants well-nourished with S as a strategy for phytoextraction. Plant Physiology and Biochemistry, v. 121, p. 48-57, DEC 2017. (15/21562-2, 14/18735-0, 14/16731-7)

SILVEIRA RABELO, FLAVIO HENRIQUE; LUX, ALEXANDER; ROSSI, MONICA LANZONI; MARTINELL, ADRIANA PINHEIRO; CUYPERS, ANN; LAVRES, JOSE. Adequate S supply reduces the damage of high Cd exposure in roots and increases N, S and Mn uptake by Massai grass grown in hydroponics. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 148, p. 35-46, APR 2018. (15/21562-2, 14/18735-0, 14/16731-7)

SILVEIRA RABELO, FLAVIO HENRIQUE; FERNIE, ALISDAIR ROBERT; NAVAZAS, ALEJANDRO; BORGO, LUCELIA; KEUNEN, ELS; DE ALCANTARA DA SILVA, BERENICE KUSSUMOTO; CUYPERS, ANN; LAVRES, JOSE. A glimpse into the effect of sulfur supply on metabolite profiling, glutathione and phytochelatins in Panicum maximum cv. Massai exposed to cadmium. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 151, p. 76-88, JUL 2018. (15/21562-2, 14/18735-0, 14/16731-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RABÊLO, Flávio Henrique Silveira. Sulfur supply as a sustainable technology for phytoextraction: its effects on cadmium uptake and detoxification in Panicum maximum Jacq. cv. Massai. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB) Piracicaba.