Structural and functional dynamics of heparin sulfate

• Grant number: 15/08782-3
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2015
• End date: June 30, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Marcelo Andrade de Lima
• Grantee: Marcelo Andrade de Lima
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Edwin Alexander Yates ; Helena Bonciani Nader

Abstract

Heparan sulfate (HS), a sulfated linear carbohydrate which decorates the cell surface and extracelullar matrix, are ubiquitously distributed throughout the animal kingdom and represent a key regulator of biological processes as well as a vast, largely untapped, reservoir of potential therapeutic agents. Dynamic temporal and spatial variations in HS structure underpin the concept of the 'heparanome', and a complex HS interactome. Understanding its structure, composition and biosynthesis is vital for comprehensive structure-function parallel of HS-protein interactions. In this project, we will investigate the structure of HS in different relevant biological models in order to understand their possible biosynthetic pathways to map HS structures relevant to biological processes. Furthermore, using spectroscopic and stopped-flow techniques, we will investigate the extent and nature of the specificity of HS-protein interactions in a conformational perspective with no inherent bias. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVALHEIRO, R. P.; LIMA, M. A.; JARROUGE-BOUCAS, T. R.; VIANA, G. M.; LOPES, C. C.; COULSON-THOMAS, V. J.; DREYFUSS, J. L.; YATES, E. A.; TERSARIOL, I. L. S.; NADER, H. B.. Coupling of vinculin to F-actin demands Syndecan-4 proteoglycan. MATRIX BIOLOGY, v. 63, p. 23-37, NOV 2017. (15/08782-3, 15/03964-6)

GHEZZI, SILVIA; COOPER, LYNSAY; RUBIO, ALICIA; PAGANI, ISABEL; CAPOBIANCHI, MARIA ROSARIA; IPPOLITO, GIUSEPPE; PELLETIER, JULIEN; MENEGHETTI, MARIA CECILIA Z.; LIMA, MARCELO A.; SKIDMORE, MARK A.; et al. Heparin prevents Zika virus induced-cytopathic effects in human neural progenitor cells. Antiviral Research, v. 140, p. 13-17, APR 2017. (15/08782-3)

DE GODOY, CARLOS M. G.; VASQUES, ENIO R.; CARICATI-NETO, AFONSO; TAVARES, JOSE G. P.; ALVES, BEATRIZ J.; DUARTE, JULIANA; MIRANDA-FERREIRA, REGIANE; LIMA, MARCELO A.; NADER, HELENA B.; DOS SANTOS TERSARIOL, IVARNE L.. Heparin Oligosaccharides Have Antiarrhythmic Effect by Accelerating the Sodium-Calcium Exchanger. FRONTIERS IN CARDIOVASCULAR MEDICINE, v. 5, JUN 7 2018. (15/08782-3, 15/03964-6)