| Grant number: | 13/19441-7 |
| Support Opportunities: | Regular Research Grants |
| Start date: | September 01, 2015 |
| End date: | August 31, 2017 |
| Field of knowledge: | Health Sciences - Medicine |
| Principal Investigator: | Luís Fernando de Macedo Brígido |
| Grantee: | Luís Fernando de Macedo Brígido |
| Host Institution: | Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil |
| Associated researchers: | Carmem Aparecida de Freitas Oliveira ; Elaine Monteiro Matsuda ; Luana Portes Ozório Coelho ; Mariza Vono Tancredi |
Abstract
The high rate of replication associated with low fidelity during the retrotranscription makes HIV an organism of extraordinary evolutionary speed, with some characteristics present in the early phase of infection, not observed in chronic, persistent phase, where most patients are identified. The study of this phase of infection is instrumental in epidemiological surveillance , in monitoring the emergence of resistance to antiretroviral drugs in the support of clinical management and can contribute to the understanding of viral pathogenesis and development of new prevention materials or treatment. The isolation and characterization of HIV are needed for better understanding of the characteristics of the relevant incident infection. The study proposes the characterization of HIV-1 from up to 320 patients with acute or recent infection, defined from epidemiological, clinical, viral and serological information. The clinical follow-up of incident cases of these documents, and their compared characteristics of patients in chronic phase, may help in understanding the molecular evolution of the virus in the patient newly infected and understanding the role of aspects of the evolution of viral diversity in the pathogenesis of HIV-1. The purpose of this study to identify and analyze virus from incident cases at different clinical sites where individuals receive the diagnose HIV infection of those identified for presenting symptoms consistent with acute infection. The presence of primary genotypic resistance will studied, with prioritization of estimates related to new classes of medications. The viral genome also be assayed for viral tropism and cloning, and sequencing of the second generation of selected cases allow a breakdown of viral diversity, as replacements nucleotide in relevant epitopes that still occur in this initial phase of disease. Isolates were coming in from volunteers will also be obtained. Prospective biological material from volunteers during clinical follow-up at collaborating units will allow additional molecular and immunological analyzes Longitudinal and its association with disease progression, allowing both to evaluate the role of these laboratory parameters in the prognosis of infection as validate and adjust the parameters described as markers of recent infection in patients from our epidemic reality, thus contributing to enhance methodologies to estimate the incidence of infection by HIV-1 and to the understand the local epidemic dynamics. (AU)
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