Reprogramming the purine metabolism of Bacillus subtilis employing sRNA-technology

• Grant number: 14/17564-7
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: September 01, 2015
• End date: November 30, 2020
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Danielle Biscaro Pedrolli
• Grantee: Danielle Biscaro Pedrolli
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 18/00507-1 - Modularity assays of the autoinduction system for gene expression constructed in Bacillus subtilis, BP.IC; 17/02594-6 - In vivo characterization of the regulatory activity of Bacillus subtilis purine riboswitches , BP.IC

Abstract

The currently employed tools to redirect metabolic flux through a specific pathway are deletion and/or insertion of regulatory sequences or genes in the bacterial chromosome. These methods permanently modify the pathway perturbing the metabolic balance during the lag growth phase, what generally extends this process and also decreases cell viability. This project proposes the construction of a new model for controlling the purine metabolism in Bacillus subtilis aiming to reversibly increase the carbon flux through it. The genes of the purine metabolism in B. subtilis are controlled by five different riboswitches whose activity can be in principle modulated by a single sRNA (small non-regulatory RNA). The goal is to exemplary model the well-studied purine metabolism in this industrially relevant bacterium and to demonstrate that the sRNA technology is actually suitable for metabolic engineering of microbial production strains. The advantage of this innovative control concept is that gene expression can be turned on but also turned off as needed. The sRNA control system will be coupled to an autocatalytic induction system (lux-system of Vibrio fischeri), which will be able to self-monitoring. The new concept is broadly applicable and could be an innovative element in the portfolio of modern synthetic biology. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDROLLI, DANIELLE B.; RIBEIRO, NATHAN V.; SQUIZATO, PATRICK N.; DE JESUS, VICTOR N.; COZETTO, DANIEL A.. Encineerinc Microolal Livinc Thera The Synthetic Bolocy Tooloox. TRENDS IN BIOTECHNOLOGY, v. 37, n. 1, p. 100-115, JAN 2019. (14/17564-7)

LINS, MILCA RACHEL DA COSTA RIBEIRO; AMORIM, LAURA ARAUJO DA SILVA; CORREA, GRACIELY GOMES; PICAO, BRUNO WILLIAN; MACK, MATTHIAS; CERRI, MARCEL OTAVIO; PEDROLLI, DANIELLE BISCARO. Targeting riboswitches with synthetic small RNAs for metabolic engineering. METABOLIC ENGINEERING, v. 68, p. 59-67, NOV 2021. (20/08699-7, 14/17564-7)

TAVARES, LEONARDO F.; RIBEIRO, NATHAN V.; ZOCCA, VITORIAF. B.; CORREA, GRACIELY G.; AMORIM, LAURA A. S.; LINS, MILCA R. C. R.; PEDROLLI, DANIELLE B.. Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit. ACS SYNTHETIC BIOLOGY, v. 12, n. 10, p. 7-pg., 2023-09-29. (20/16058-1, 14/17564-7, 18/04703-0)

CORREA, GRACIELY GOMES; DA COSTA RIBEIRO LINS, MILCA RACHEL; SILVA, BRUNA FERNANDES; DE PAIVA, GABRIELA BARBOSA; BERTOLAZZI ZOCCA, VITORIA FERNANDA; RIBEIRO, NATHAN VINICIUS; PICHELI, FLAVIO PEREIRA; MACK, MATTHIAS; PEDROLLI, DANIELLE BISCARO. Dataset for supporting a modular autoinduction device for control of gene expression in Bacillus subtilis. DATA IN BRIEF, v. 31, p. 8-pg., 2020-08-01. (14/17564-7)

CORREA, GRACIELY GOMES; DA COSTA RIBEIRO LINS, MILCA RACHEL; SILVA, BRUNA FERNANDES; DE PAIVA, GABRIELA BARBOSA; BERTOLAZZI ZOCCA, VITORIA FERNANDA; RIBEIRO, NATHAN VINICIUS; PICHELI, FLAVIO PEREIRA; MACK, MATTHIAS; PEDROLLI, DANIELLE BISCARO. A modular autoinduction device for control of gene expression in Bacillus subtilis. METABOLIC ENGINEERING, v. 61, p. 9-pg., 2020-09-01. (14/17564-7)

ZOCCA, VITORIA FERNANDA BERTOLAZZI; CORREA, GRACIELY GOMES; LINS, MILCA RACHEL DA COSTA RIBEIRO; DE JESUS, VICTOR NUNES; TAVARES, LEONARDO FERRO; AMORIM, LAURA ARAUJO DA SILVA; KUNDLATSCH, GUILHERME ENGELBERTO; PEDROLLI, DANIELLE BISCARO. The CRISPR toolbox for the gram-positive model bacterium Bacillus subtilis. CRITICAL REVIEWS IN BIOTECHNOLOGY, OCT 2021. (18/24733-0, 14/17564-7, 20/16058-1)

SILVA, BRUNA F.; CORREA, GRACIELY G.; ZOCCA, VITORIA F. B.; PICHELI, FLAVIO P.; LINS, MILCA R. C. R.; PEDROLLI, DANIELLE B.. Expanding the Functionality of an Autoinduction Device for Repression of Gene Expression in Bacillus subtilis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 1, p. 6-pg., 2023-01-01. (14/17564-7)

PEDROLLI, DANIELLE B.; RIBEIRO, NATHAN V.; SQUIZATO, PATRICK N.; DE JESUS, VICTOR N.; COZETTO, DANIEL A.. Engineering Microbial Living Therapeutics: The Synthetic Biology Toolbox. TRENDS IN BIOTECHNOLOGY, v. 37, n. 1, p. 16-pg., 2019-01-01. (14/17564-7)