Vitrification of Astyanax altiparanae (Garutti & Britski, 2000) embryos

• Grant number: 15/10115-5
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2015
• End date: August 31, 2017
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Alexandre Ninhaus Silveira
• Grantee: Alexandre Ninhaus Silveira
• Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil
• Associated researchers: Cristiéle da Silva Ribeiro ; Rosicleire Veríssimo Silveira

Abstract

The development of gamete cryopreservation techniques and fish embryos is of great value to the formation of gene banks, serving as a basis for restocking studies, intensive fish farming, breeding programs and aid the preservation of species at risk of extinction . Current methods of cryopreservation of neotropical fish embryos, are still ineffective since run into problems related to sensitivity to cold, to cryoprotectants and the low permeability of the membranes, which can lead to loss of functional and structural integrity of the embryos, after application the cooling process, freezing and thawing. A major component of the plasma membrane, the fatty acids, plays a key role both in gametogenesis process, as in the structural formation of embryos and are fundamental in controlling the permeability of cell membranes. Thus, diets with high levels of highly unsaturated fatty acids (HUFA) to breeding, can provide a change in the composition of the plasma membrane of the gametes and the embryo, and may enable a change in the permeability of the sperm and embryonic tissues. Thus, contextualizing the methodological problem of teleost embryo cryopreservation process, and the importance of fatty acids, as changeable components and may assist in the success of these processes, this project aims to the incorporation of fatty acids in the membranes of embryos through incorporation oil with high concentrations of HUFA in the feed parental aimed at increasing the permeability to cryoprotectant, and this can increase the resistance to cryopreservation protocols, and the development of a process vitrifying Astyanax altiparanae embryos important fish species in the Brazilian aquaculture setting. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COELHO, GEOVANNA CARLA ZACHEO; DA SILVA COSTA, RAPHAEL; PORTO-FORESTI, FABIO; VERISSIMO-SILVEIRA, ROSICLEIRE; NINHAUS-SILVEIRA, ALEXANDRE. Comparative post-hatching ontogeny of Pseudoplatystoma reticulatum (Eigenmann & Eigenmann 1889) and Leiarius marmoratus (Gill, 1870) and their hybrid. Journal of Applied Ichthyology, v. 37, n. 3, FEB 2021. (15/10115-5)

COSTA, RAPHAEL DA SILVA; SILVA SOUZA, FABRICIO MARCAL; SENHORINI, JOSE AUGUSTO; BASHIYO-SILVA, CRISTIANE; VERISSIMO-SILVEIRA, ROSICLEIRE; ZACHEO COELHO, GEOVANNA CARLA; SIQUEIRA-SILVA, DIOGENES HENRIQUE; RIBEIRO, CRISTIELE DA SILVA; NINHAUS-SILVEIRA, ALEXANDRE. Fatty acid influence on Prochilodus lineatus (Characiformes, Prochilodontidae) embryo cryopreservation parameters.. AQUACULTURE RESEARCH, v. 49, n. 8, p. 2714-2722, AUG 2018. (15/10115-5, 13/02588-5)