Impact of translational regulation into the neural differentiation

• Grant number: 14/21704-9
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: September 01, 2015
• End date: October 31, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Mário Henrique Bengtson
• Grantee: Mário Henrique Bengtson
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: André Fujita ; Katlin Brauer Massirer ; Roberto Hirochi Herai
• Associated scholarship(s): 19/00154-4 - Impact of translational regulation into the neural differentiation, BP.TT; 16/23120-0 - Identification of factors responsible for translational regulation in neuronal differentiation, BP.DD; 15/21433-8 - Identification of molecular pathways translationally regulated in neuronal differentiation, BP.DD

Abstract

During cell differentiation, several mechanisms are used by cells to warranty that genes are expressed in the right time and in the right level, modulating the multiple pathways involved on this complex process.One of these mechanisms controls which specific mRNAs and in what rate will be translated by the ribosome, and it is known as translation control of gene expression. Since some time ago, it has being known that this mechanism should be fundamental to cell functioning, considering that protein expression poorly correlates with mRNA expression. Despite this, we lack fundamental understanding of which are all the factor that participate on this process, how it operates and which pathways are modulated by it.On this project, we propose to try to understand how translation control participates in the neural differentiation of stem cells. We want to identify genes and pathways regulated by this process and the possible proteins/miRNA participating in the mechanism. To this aim, we propose to compare the global levels of each mRNA expressed (RNAseq) with global levels of each corresponding mRNA bound to ribosomes (Riboseq) in different time points of neural differentiation induction. This would allow for instances, the identification of genes whose mRNA expression doesn't change, but whose translation rate is controlled during differentiation. The miRNA and RNA biding proteins expression levels will be compared in the same time points in search of possible players in the process. We will use cell and biochemistry assays and bioinformatics to determine the impact of the translation control on the differentiation process. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LINS, ERICO MORETO; OLIVEIRA, NATASSIA CRISTINA MARTINS; REIS, OSVALDO; FERRASA, ADRIANO; HERAI, ROBERTO; MUOTRI, ALYSSON R.; MASSIRER, KATLIN BRAUER; BENGTSON, MARIO HENRIQUE. Genome-wide translation control analysis of developing human neurons. MOLECULAR BRAIN, v. 15, n. 1, p. 15-pg., 2022-06-15. (14/21704-9, 14/50897-0, 15/21433-8)

MIGITA, NATACHA AZUSSA; JOTTA, PATRICIA YOSHIOKA; DO NASCIMENTO, NATALIA PAIVA; VASCONCELOS, VICTOR SANDE; CENTODUCATTE, GABRIEL LOPES; MASSIRER, KATLIN BRAUER; DE AZEVEDO, AMILCAR CARDOSO; BRANDALISE, SILVIA REGINA; YUNES, JOSE ANDRES. Classification and genetics of pediatric B-other acute lymphoblastic leukemia by targeted RNA sequencing. BLOOD ADVANCES, v. 7, n. 13, p. 15-pg., 2023-07-11. (14/21704-9, 14/50897-0)

MARTINS OLIVEIRA, NATASSIA CRISTINA; LINS, ERICO MORETO; MASSIRER, KATLIN BRAUER; BENGTSON, MARIO HENRIQUE. Translational Control during Mammalian Neocortex Development and Postembryonic Neuronal Function. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, v. 114, n. SI, p. 36-46, JUN 2021. (15/21433-8, 14/21704-9)

SACHAMITR, PATTY; HO, JOLENE C.; CIAMPONI, FELIPE E.; BA-ALAWI, WAIL; COUTINHO, FIONA J.; GUILHAMON, PAUL; KUSHIDA, MICHELLE M.; CAVALLI, FLORENCE M. G.; LEE, LILIAN; RASTEGAR, NAGHMEH; et al. PRMT5 inhibition disrupts splicing and stemness in glioblastoma. NATURE COMMUNICATIONS, v. 12, n. 1, FEB 12 2021. (16/25521-1, 15/25134-5, 14/50897-0, 14/21704-9)