Adiponectin -11377C/G and +276G/T polymorphisms affect adiponectin levels but do not modify responsiveness to therapy in resistant hypertension

• Grant number: 15/10934-6
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: August 01, 2015
• End date: January 31, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Clinical Pharmacology
• Principal Investigator: Ana Paula Cabral de Faria Modolo
• Grantee: Ana Paula Cabral de Faria Modolo
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Resistant hypertension (RHTN) is a multifactorial and polygenic disease, frequently associated with obesity. Low plasma adiponectin levels, a hormone produced by the adipose tissue, were associated with RHTN. Single-nucleotide polymorphisms (SNPs) -11377C/G (rs266729) and +276G/T (rs1501299) in ADIPOQ (adiponectin gene) were associated with hypertension. This study evaluated the association between two SNPs (-11377C/G and +276G/T) and adiponectin levels in RHTN. This study comprised 109 RHTN patients genotyped for both polymorphisms. A cross-sectional study was designed to compare features of CC homozygous vs. G-allele carriers for -11377C/G and GG homozygous vs. T-allele carriers for +276G/T. Office and ambulatory BP measurements were similar among genotypes subgroups in both SNPs as well as the markers of target organ damage (arterial stiffness, left ventricular mass index and microalbuminuria). Adiponectin concentrations were significantly higher in CC compared to G-carrier for -11377C/G (CC=7.0 (4.0-10.2) vs. G allele=5.5 (2.5-7.9), p=0.04) and lower in GG compared to T-carrier for +276G/T (GG=5.3 (2.3-7.7) vs. T allele=7.1 (3.6-10.5), p=0.04). Adjusting for systolic ambulatory BP, body mass index, age, gender, race and presence of type 2 diabetes, multiple linear regression analyses revealed that the minor alleles G (beta coefficient= -0.14, SE=0.07, p=0.03) and T (beta coefficient=0.12, SE=0.06, p=0.04) were independent predictors of adiponectin. The -1377C/G and +276G/T SNPs in ADIPOQ were associated with adiponectin levels in RHTN subjects. (AU)