Multi-User Equipment approved in grant 2013/07245-9: BD FACSAria cell sorter with BD FACSAria ACDU and acessories

• Grant number: 15/12894-1
• Support Opportunities: Multi-user Equipment Program
• Start date: March 01, 2016
• End date: February 28, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Camila de Oliveira Rodini Pegoraro
• Grantee: Camila de Oliveira Rodini Pegoraro
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated research grant: 13/07245-9 - Investigation of the role of cancer stem cells and microenvironment on epithelial-to-mesenchymal transition, invasion and metastasis in oral squamous cell carcinoma, AP.JP
• EMU web page: http://www1.fob.usp.br/pesquisa/equipamentos-multiusuarios/bd-facsaria-fusion/
• Type of equipment: Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
• Manufacturer: BD Biosciences
• Model: FACSAria

Abstract

This Multi-User Equipment (MUE) is a Fluorescent Activated Cell Sorter with 3 lasers (blue, red and near UV) capable of analyzing up to 12 parameters simultaneously (10 colors, SSC and FSC) and separating up to 4 different cell populations. This configuration can be expanded to meet future needs as well as the platform can be upgraded instead of purchasing new systems, what makes the acquisition of the equipment a long term investment. Besides being essential to the development of the present research project, its use can be extended to all researchers from FOB as well as to others. In fact, several researchers from FOB are interested on using the equipment in their next studies. This MUE enables the realization of research protocols of cell sorting with high quality and sterile condition, independently of the purpose of the study and research field, offering high quality support for national or international researchers. There are only 3 cells sorters in São Paulo State and the installation of the MUE in Bauru can easily reach the whole countryside. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CAMARGO, MARCELA RODRIGUES; FRAZON, TALITA FONSECA; INACIO, KELLY KARINA; SMIDERLE, FHERNANDA RIBEIRO; AMOR, NADIA GHINELLI; DIONISIO, THIAGO JOSE; SANTOS, CARLOS FERREIRA; RODINI, CAMILA OLIVEIRA; LARA, VANESSA SOARES. Ganoderma lucidum polysaccharides inhibit in vitro tumorigenesis, cancer stem cell properties and epithelial-mesenchymal transition in oral squamous cell carcinoma. Journal of Ethnopharmacology, v. 286, p. 15-pg., 2022-03-25. (13/07245-9, 17/01212-2, 18/04203-7, 15/12894-1)