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NANOCEL - development of enzyme mixtures for the preparation of nanocelluloses by a biohibrid process

Grant number: 15/02862-5
Support type:Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
Duration: December 01, 2015 - November 30, 2020
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal Investigator:Valdeir Arantes
Grantee:Valdeir Arantes
Home Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
Associated scholarship(s):15/00397-3 - Study on the action of endoglucanases in the production of nanocrystalline cellulose, BP.PD


Nanocellulose is a new class of renewable nanomaterial that can lead to several benefits in areas such as biomedical, automotive, aerospace, pharmaceuticals, chemicals, and electronics. The traditional production processes involves severe chemical or mechanical treatment steps, which have several technical, economic and environmental limitations. The recent proposal to add or replace one of these steps by an enzymatic treatment is an innovative and promising alternative, but still with very limited efficiency since the enzyme preparations typically used are developed and optimized for use in other industrial sectors (i.e. food, textile, pulp and paper). Therefore, to increase the efficiency of the enzymatic treatment, auxiliary pre-treatment steps are necessary for production of cellulose nanofibrillated (CNF), and a high amount of enzyme is necessary in the production of cellulose nanocrystalline (CNC), which all results in high cost. In this project it is proposed to identify essential enzymes and proteins and their properties more suitable for application in the production of nanocelluloses and using these enzymes and proteins to develop and optimize specific enzymatic preparations with high efficiency to be used in the production of CNC and CNF in a sustainably and more technoeconomically feasible manner. The identification of these biochemical agents and their properties will be obtained through the elucidation of the mode of action and interaction of various biochemical systems (hydrolytic, oxidative, amorphogenesis-inducing) in the production of CNC and CNF. A novel adsorption-based enzymatic reaction system will be developed to enable the production of nanocellulose in a Biorefinery concept. The development of enzymatic preparations and adsorptive enzymatic reaction process will also allow assessing the techno-economic feasibility of integrating the production of nanocelluloses (as a high value co-product) with the cellulosic ethanol production process in a Biorefinery concept. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA, BARBARA; ARANTES, VALDEIR. Production of cellulose nanocrystals integrated into a biochemical sugar platform process via enzymatic hydrolysis at high solid loading. INDUSTRIAL CROPS AND PRODUCTS, v. 152, SEP 15 2020. Web of Science Citations: 0.
ARANTES, VALDEIR; DIAS, ISABELLA K. R.; BERTO, GABRIELA L.; PEREIRA, BARBARA; MAROTTI, BRAZ S.; NOGUEIRA, CARLAILE F. O. The current status of the enzyme-mediated isolation and functionalization of nanocelluloses: production, properties, techno-economics, and opportunities. Cellulose, JUL 2020. Web of Science Citations: 0.
SIQUEIRA, GERMANO A.; DIAS, ISABELLA K. R.; ARANTES, VALDEIR. Exploring the action of endoglucanases on bleached eucalyptus kraft pulp as potential catalyst for isolation of cellulose nanocrystals. International Journal of Biological Macromolecules, v. 133, p. 1249-1259, JUL 15 2019. Web of Science Citations: 0.
BERTO, GABRIELA L.; ARANTES, VALDEIR. Kinetic changes in cellulose properties during defibrillation into microfibrillated cellulose and cellulose nanofibrils by ultra-refining. International Journal of Biological Macromolecules, v. 127, p. 637-648, APR 15 2019. Web of Science Citations: 0.
COELHO DE CARVALHO BENINI, KELLY CRISTINA; CORNELIS VOORWALD, HERMAN JACOBUS; HILARIO CIOFFI, MARIA ODILA; REZENDE, MIRABEL CERQUEIRA; ARANTES, VALDEIR. Preparation of nanocellulose from Imperata brasiliensis grass using Taguchi method. Carbohydrate Polymers, v. 192, p. 337-346, JUL 15 2018. Web of Science Citations: 14.

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Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1020180684850 - Universidade de São Paulo (USP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - September 2018, 12

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1020180747843 - Universidade de São Paulo (USP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - November 2018, 29