"Quantification of the parasitic load of the lesions of Balb/c mice treated with amphotericin B deoxycholate associated with chylomicron-like artificial lipid nanoemulsion (QMA-AB)"

• Grant number: 15/10038-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2016
• End date: August 31, 2018
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Valdir Sabbaga Amato
• Grantee: Valdir Sabbaga Amato
• Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

American cutaneous leishmaniasis (ACL), an infectious disease, not contagious, of vectorial transmission with heteroxenic cycle, which affects the skin and mucous membranes. The drugs currently available for treatment of the disease are poor due to their limited efficacy and side effects, besides the high cost and the resistance against protozoa accumulate these drugs. Lipid formulations of amphotericin B deoxycholate (AB) is presented as a breakthrough in the treatment of (LTA). In studies conducted by the group of the Heart Institute of the Medical School and USP's Faculty of Pharmacy showed that amphotericin B deoxycholate (AB) may be associated with the lipid nanoemulsions (LDE) (Maranhão, 2008). Molecular methods, real-time PCR (qPCR) shows the advantages of using computerized data, do not require specialized technical in parasitology, identify and quantify the species of the parasite, measure the seasonal variations of the parasite in wild host, and perhaps to determine the effectiveness of drugs in human and experimental leishmaniasis (Ministry 2010). Therefore, it is intended to evaluate the efficacy and tolerability of the use of amphotericin B deoxycholate associated with chylomicron-like artificial lipid nanoemulsion (QMA-AB) against amphotericin B in deoxycholate treatment of leishmaniasis (ACL) in experimental models and quantify the parasitic load molecular technique for real time PCR (qPCR ). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, REGINA MAIA; MARANHAO, RAUL CAVALCANTE; TAVARES, ELAINE RUFO; FILIPPIN-MONTEIRO, FABIOLA BRANCO; NICODEMO, ANTONIO CARLOS; MORIKAWA, ALEKSANDRA TIEMI; YAMASHIRO KANASHIRO, EDITE HATSUMI; AMATO, VALDIR SABBAGA. Lipid nanoparticles for amphotericin delivery in the treatment of American tegumentary leishmaniasis. DRUG DELIVERY AND TRANSLATIONAL RESEARCH, v. 10, n. 2, p. 10-pg., 2019-11-07. (15/10038-0)

DE SOUZA, REGINA MAIA; MARANHAO, RAUL CAVALCANTE; TAVARES, ELAINE RUFO; FILIPPIN-MONTEIRO, FABIOLA BRANCO; NICODEMO, ANTONIO CARLOS; MORIKAWA, ALEKSANDRA TIEMI; YAMASHIRO KANASHIRO, EDITE HATSUMI; AMATO, VALDIR SABBAGA. Lipid nanoparticles for amphotericin delivery in the treatment of American tegumentary leishmaniasis. DRUG DELIVERY AND TRANSLATIONAL RESEARCH, NOV 2019. (15/10038-0)