Impact of parenteral administration of vitamins D and E on insulin sensitivity and on oxidative stress in ewes in the peripartum period

• Grant number: 14/17297-9
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2016
• End date: March 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Maria Claudia Araripe Sucupira
• Grantee: Maria Claudia Araripe Sucupira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

To evaluate the effect of parenteral supplementation with vitamins D and E in the peripartum period in glucose tolerance test and the oxidative metabolism, 30 ewes, adult and healthy crossbred Santa Inês and Dorper will be used. After pregnancy diagnosis and detection of the number of fetuses, these will be divided into three groups of ten animals that receive in the 100th (hundredth) day of gestation, a dose, by deep intramuscular injection of 2 mL of oil vehicle (control group -GC); 70,000 IU / kg BW of vitamin D3 (cholecalciferol) (GD-treated group); or 60UI/kg PV of vitamin E (alpha-tocopherol) (GE-treated group) and will be submitted to the glucose tolerance test (GTT) four weeks before lambing. Blood samples will be collected prior the dose of vitamin (M0); four weeks before delivery (M1); two weeks before delivery (M2); at delivery (M3); one week after delivery (M4); two weeks after birth (M5); and four weeks after birth (M6). Insulin, cortisol, glucose, beta hydroxybutyrate (BHB), non-esterified fatty acids (AGNES), urea, creatinine, total calcium, ionic calcium and total protein will be determinated. Oxidative metabolism will be determined ferric reducing ability of plasma (HRFP), activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and test for thiobarbituric acid reactive substances (Thiobarbituric acid reactive substances - TBARS). During the trial period we will determine the concentrations of vitamins D and E, in the moments above descript and in the day of TTG. (AU)