Fabrication of nanometric probes for the study of biochemical processes by using Scanning Electrochemical Microscopy

• Grant number: 15/20776-9
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2016
• End date: May 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Mauro Bertotti
• Grantee: Mauro Bertotti
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant(s): 16/50020-6 - Fabrication and characterisation of nanoelectrochemical sensors for the study of single cells and single nanoparticles, AP.R SPRINT

Abstract

This research project aims at the development of electrodes with structurally microscopic dimensions, i.e. both the active area of the electrode (conductive fiber) as the insulation coating have dimensions in the micro or nanometer scale. These tips will be employed as sensors coupled to an electrochemical microscope equipment to get high resolution information on the transport of chemical species into cells, tissues and synthetic and biological membranes. The experimental data will be fitted in accordance with existing theories for the diffusion of chemical species in solution, pores and membranes. The technique will be used in the monitoring of mitochondrial oxygen consumption, quantification of reactive oxygen/nitrogen and tioredoxins in macrophages subjected to inflammatory stimuli and studies on the transport of glutathione through cellular membranes. The ultimate goal is to create enough and suitable infrastructure conditions for the full establishment of a reference laboratory in Brazil to carry out experiments with Scanning Electrochemical Microscopy (SECM). (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MELONI, GABRIEL N.; BERTOTTI, MAURO. 3D printing scanning electron microscopy sample holders: A quick and cost effective alternative for custom holder fabrication. PLoS One, v. 12, n. 7, JUL 28 2017. (15/20776-9)

SANTOS, CARLA SANTANA; BANNITZ-FERNANDES, RENATA; LIMA, ALEX S.; TAIRUM, CARLOS A.; MALAVAZI, IRAN; NETTO, LUIS E. S.; BERTOTTI, MAURO. Monitoring H2O2 inside Aspergillus fumigatus with an Integrated Microelectrode: The Role of Peroxiredoxin Protein Prx1. Analytical Chemistry, v. 90, n. 4, p. 2587-2593, FEB 20 2018. (13/14955-2, 15/02095-4, 15/20776-9, 14/22396-6)

CATOTA SAENZ, HENRY STEVEN; PATRICIO HERNANDEZ-SARAVIA, LUCAS; SELVA, JESSICA S. G.; SUKERI, ANANDHAKUMAR; JAVIER ESPINOZA-MONTERO, PATRICIO; BERTOTTI, MAURO. Electrochemical dopamine sensor using a nanoporous gold microelectrode: a proof-of-concept study for the detection of dopamine release by scanning electrochemical microscopy. Microchimica Acta, v. 185, n. 8, AUG 2018. (14/15215-5, 15/20776-9)

CRITELLI, RENAN A. J.; SUMODJO, PAULO T. A.; BERTOTTI, MAURO; TORRESI, ROBERTO M.. Influence of glycine on Co electrodeposition: IR spectroscopy and near-surface pH investigations. Electrochimica Acta, v. 260, p. 762-771, JAN 10 2018. (15/20776-9, 15/26308-7)

SUKERI, ANANDHAKUMAR; BERTOTTI, MAURO. Electrodeposited honeycomb-like dendritic porous gold surface: An efficient platform for enzyme-free hydrogen peroxide sensor at low overpotential. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 805, p. 18-23, NOV 15 2017. (14/15215-5, 15/20776-9)

CRITELLI, RENAN A. J.; BERTOTTI, MAURO; TORRESI, ROBERTO M.. Probe effects on concentration profiles in the diffusion layer: Computational modeling and near-surface pH measurements using microelectrodes. Electrochimica Acta, v. 292, p. 511-521, DEC 1 2018. (15/26308-7, 15/20776-9)

SUKERI, ANANDHAKUMAR; BERTOTTI, MAURO. Nanoporous Gold Surface: An Efficient Platform for Hydrogen Evolution Reaction at Very Low Overpotential. Journal of the Brazilian Chemical Society, v. 29, n. 2, p. 226-231, FEB 2018. (14/15215-5, 15/20776-9)

ALAN R. F. LIMA; ALEX S. LIMA; GABRIEL N. MELONI; CARLA S. SANTOS; MAURO BERTOTTI. Perovskite-Type Oxides La0.6M0.4Ni0.6Cu0.4O3 (M = Ag, Ba, Ce) towards the Oxygen Reduction Reaction (ORR) in Alkaline Medium: Structural Aspects and Electrocatalytic Activity. Journal of the Brazilian Chemical Society, v. 32, n. 3, p. 665-674, 2021-03-01. (14/22396-6, 15/02095-4, 15/20776-9)

SUKERI, ANANDHAKUMAR; LIMA, ALEX S.; BERTOTTI, MAURO. Development of non-enzymatic and highly selective hydrogen peroxide sensor based on nanoporous gold prepared by a simple unusual electrochemical approach. Microchemical Journal, v. 133, p. 149-154, JUL 2017. (14/15215-5, 15/20776-9)

ORELLANA JARAMILLO, DARIO XAVIER; SUKERI, ANANDHAKUMAR; SARAVIA, LUCAS P. H.; ESPINOZA-MONTERO, PATRICIO JAVIER; BERTOTTI, MAURO. Nanoporous Gold Microelectrode: A Novel Sensing Platform for Highly Sensitive and Selective Determination of Arsenic (III) using Anodic Stripping Voltammetry. Electroanalysis, v. 29, n. 10, p. 2316-2322, OCT 2017. (14/15215-5, 15/20776-9)

BARROSO, IGNACIO G.; SANTOS, CARLA S.; BERTOTTI, MAURO; FERREIRA, CLELIA; TERRA, WALTER R.. Molecular mechanisms associated with acidification and alkalization along the larval midgut of Musca domestica. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 237, NOV 2019. (15/20776-9, 17/08103-4)

KUMAR, ABHISHEK; GONCALVES, JOSUE M.; SUKERI, ANANDHAKUMAR; ARAKI, KOITI; BERTOTTI, MAURO. Correlating surface growth of nanoporous gold with electrodeposition parameters to optimize amperometric sensing of nitrite. SENSORS AND ACTUATORS B-CHEMICAL, v. 263, p. 237-247, JUN 15 2018. (14/15215-5, 15/20776-9, 16/07461-1)

GONCALVES, JOSUE M.; MATIAS, TIAGO A.; SARAVIA, LUCAS P. H.; NAKAMURA, MARCELO; BERNARDES, JULIANA S.; BERTOTTI, MAURO; ARAKI, KOITI. Synergic effects enhance the catalytic properties of alpha-Ni(OH)(2)-FeOCPc@rGO composite for oxygen evolution reaction. Electrochimica Acta, v. 267, p. 161-169, MAR 20 2018. (15/20776-9)

LIMA, ALAN R. F.; LIMA, ALEX S.; MELONI, GABRIEL N.; SANTOS, CARLA S.; BERTOTTI, MAURO. Perovskite-Type Oxides La_0.6M_0.4Ni_0.6Cu_0.4O₃ (M = Ag, Ba, Ce) towards the Oxygen Reduction Reaction (ORR) in Alkaline Medium: Structural Aspects and Electrocatalytic Activity. Journal of the Brazilian Chemical Society, v. 32, n. 3, p. 10-pg., 2021-03-01. (15/20776-9, 15/02095-4, 14/22396-6)