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Fabrication of nanosensors to be used in scanning electrochemical microscopy and further application in biological systems (single-cell analysis)

Grant number: 15/02095-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): August 01, 2015
Effective date (End): July 31, 2019
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Mauro Bertotti
Grantee:Alex da Silva Lima
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):17/00132-5 - The use of micro and nanosensors in the study of neural signaling, BE.EP.PD


Macrophages are important cells component of the human immune system. These cells produce and release reactive oxygen and reactive nitrogen species after inflammatory activation and, consequently, they can oxidize distinct thiol groups in proteins. Accordingly, information on the concentration of these species can help to understand their function in signal transduction pathways. Therefore, this project aims at the fabrication of nanosensors to be used in scanning electrochemical microscopy (SECM) and further application of such probes to get information on reactive oxygen/nitrogen species and thioredoxin proteins in macrophages after an inflammatory activation. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANTOS, CARLA SANTANA; BANNITZ-FERNANDES, RENATA; LIMA, ALEX S.; TAIRUM, CARLOS A.; MALAVAZI, IRAN; NETTO, LUIS E. S.; BERTOTTI, MAURO. Monitoring H2O2 inside Aspergillus fumigatus with an Integrated Microelectrode: The Role of Peroxiredoxin Protein Prx1. Analytical Chemistry, v. 90, n. 4, p. 2587-2593, FEB 20 2018. Web of Science Citations: 3.
LIMA, ALEX S.; PRIETO, KATIA R.; SANTOS, CARLA S.; VALERIO, HELLEN PAULA; GARCIA-OCHOA, EVELYN Y.; HUERTA-ROBLES, AURORA; BELTRAN-GARCIA, MIGUEL J.; DI MASCIO, PAOLO; BERTOTTI, MAURO. In-vivo electrochemical monitoring of H2O2 production induced by root-inoculated endophytic bacteria in Agave tequilana leaves. BIOSENSORS & BIOELECTRONICS, v. 99, p. 108-114, JAN 15 2018. Web of Science Citations: 9.

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