Strategies to improve the regeneration of the calcaneal tendon

• Grant number: 15/24087-3
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2016
• End date: March 31, 2018
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Ii-Sei Watanabe
• Grantee: Ii-Sei Watanabe
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Tendons have been described like dense regular connective tissue that attaches muscle to bone. Their principal function is transferring contractile forces from muscle to bone and then generate movement. Calcaneal tendon (Achilles) is one of the most frequently injured since the incidence of rupture in North America is 5.5 to 9.9/100,000 habitants and 6.8 to 18/100,000 in Europe. It occurs mostly in men and is more frequent between the third and fourth decade of life. There are many suture methods, however, Kessler-Tajima suture with nodes between the stumps is widely used to avoid the bottleneck of microcirculation. After surgery, the patients return to their activity on approximately 85 days, but frequently may range up to 270 days. Aim: Apply F1 protein in the injured tendon with the intention of decrease repair time, even as analyze to influence of the suture techniques in the regeneration, moreover morphological and ultrastructural alterations, of the collagen that make up tissue and capacity in transferring contractile forces from muscle to bone will be also evaluated. Methods: Sprague Dawley rats with 3 months-old will be divided into 5 experimental groups (150 total n): C group (control), K group (Kessler-Tajima), S group (simple suture technique), KP group (Kessler-Tajima plus F1 protein), SP group (simple suture plus F1 protein), 5 rats per group. All groups will be analyzed 2 and 4 weeks after surgery. Considering the few studies in the literature about this clinical problem the results obtained in the present work may contribute to understanding of suture technique influence, tissue alterations and a possible decrease in the repair time. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CURY, DIEGO PULZATTO; SCHAFER, BARBARA TAVARES; YOKOMIZO DE ALMEIDA, SONIA REGINA; DA SILVA RIGHETTI, MARTA MARIA; WATANABE, II-SEI. Application of a Purified Protein From Natural Latex and the Influence of Suture Type on Achilles Tendon Repair in Rats. AMERICAN JOURNAL OF SPORTS MEDICINE, v. 47, n. 4, p. 901-914, MAR 2019. (15/24087-3)

CURY, DIEGO PULZATTO; WATANABE, II-SEI. Collagen analysis of the Achilles tendon injured and treated with F1 protein. A study using transmission electron, polarized light microscopy and western blot.. FASEB JOURNAL, v. 31, p. 1-pg., 2017-04-01. (15/24087-3)