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Embryonic cell cultures of Rhipicephalus sanguineus (lineages tropical and temperate) for Ehrlichia canis cultivation

Abstract

The first line of embryonic tick cells established in Brazil was to Amblyomma sculptum Berlese, whose cellular products were patented (see background). This species, until recently was synonym of Amblyomma cajennense (Fabricius). The species A. cajennense is restricted to the Amazon and part of the Midwest, while A. sculptum occurs in the rest of the country, and this is the main species esponsible for transmitting R. rickettsii in its distribution area. The most frequent tick-borne diseases are Rickettsiosis, Borreliosis and to a lesser extent the Erliquioses and Protozoonoses. In the present study we suggest to standardize the culture of embryonic cells of Rhipicephalus sanguineus (Latreille), origin tropical and temperate, to obtain cell lines, and to cultivate Ehrlichia canis, as well as to analysis the proteins expressed in uninfected and infected cells. (AU)

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VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARROS-BATTESTI, DARCI MORAES; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO; MARQUES DE SOUSA, KEYLA CARSTENS; FRANZE, DANIELLA APARECIDA; LIMA-DUARTE, LEIDIANE; CIRELLI-MORAES, ANGELINA; NUNES, PABLO HENRIQUE; LABRUNA, MARCELO BAHIA; MORAES-FILHO, JONAS; et al. Successful Infection of Tick Cell Cultures of Rhipicephalus sanguineus (Tropical Lineage) with Ehrlichia canis. VECTOR-BORNE AND ZOONOTIC DISEASES, v. 18, n. 12, . (15/26209-9)
LUZZI, MAYARA DE CASSIA; LOPES DE CARVALHO, LUCAS AMOROSO; PINHEIRO, DANIEL GUARIZ; LIMA-DUARTE, LEIDIANE; CAMARGO, JAQUELINE VALERIA; KISHI, LUCIANO TAKESHI; FERNANDES, CAMILA CESARIO; MACHADO, ROSANGELA ZACARIAS; SOARES, JOAO FABIO; ANDRE, MARCOS ROGERIO; et al. Analysis on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two specific localities in Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 30, n. 3, . (15/26209-9, 20/03190-9)
LIMA-DUARTE, LEIDIANE; CAMARGO, JAQUELINE VALERIA; CASTRO-SANTIAGO, ANA CAROLINA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO; CABRAL-DE-MELLO, DIOGO CAVALCANTI; CAMARGO-MATHIAS, MARIA IZABEL; IKEDA, PRISCILA; ANHOLETO, LUIS ADRIANO; PEREIRA, MELISSA CAROLINA; et al. Establishment and characterization of a cell line (RBME-6) of Rhipicephalus (Boophilus) microplus from Brazil. TICKS AND TICK-BORNE DISEASES, v. 12, n. 5, . (15/26209-9, 20/03190-9)

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