Correlation between the biochemical parameters of Trypanosoma cruzi isolates from Chagas disease patients with different clinical forms of the disease and the pathogenesis of the disease

• Grant number: 15/24595-9
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2016
• End date: September 30, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Fernanda Ramos Gadelha
• Grantee: Fernanda Ramos Gadelha
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Eduardo de Figueiredo Peloso

Abstract

Chagas disease (CD) has different clinical forms that vary among individuals and geographic regions. CD geographic heterogeneity suggests that the genetic variability of the host, the parasite, or of both are important for the development of the distinct clinical forms of the disease. Besides that, the heterogeneity among Trypanosoma cruzi strains has been the Achile's heel for the development of a more specific therapy, and as so, less toxic to the vertebrate host. Taking this into consideration, the proposal of this project is to perform a comparative analysis of the proteomic and metabolomic profile, mitochondrial bioenergetics, calcium homeostasis and antioxidant defenses, and biological evaluation in murine model of T. cruzi isolates from CD patients with different clinical forms of the disease. These results will allow us to establish a correlation between the parasites and the different clinical forms of CD. A clinical-epidemiological profile of the patients treated on the Hospital at UNICAMP will be evaluated and will allow a discussion regarding the CD scenario within the last five years in the region. In parallel, the double knockout for cytosolic/mitochondrial tryparedoxin peroxidase (TcCMPx) and the cytosolic one (TcCPx), will allow us to widen our knowledge of the role of these peroxiredoxins in parasite survival and the possible role of TcCMPx as a chaperone. The development of this research project will bring important contributions for a better understanding of CD pathogenesis and peroxiredoxins role in parasite survival. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALCANTARA, LAURA M.; FERREIRA, THALITA C. S.; GADELHA, FERNANDA R.; MIGUEL, DANILO C.. Challenges in drug discovery targeting TriTryp diseases with an emphasis on leishmaniasis. INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE, v. 8, n. 3, p. 430-439, DEC 2018. (15/24595-9, 14/21129-4, 15/10436-6)

BRUSCATO, ANDRESSA; PEREIRA, MARIANE BARROSO; ARCHILIA, MARIANA DEGAKI; TEODORO, THASSIA MARIANE; DE ALMEIDA, EROS ANTONIO; MARTINS, LUIZ CLAUDIO; PELOSO, EDUARDO DE FIGUEIREDO; GADELHA, FERNANDA RAMOS. Using a Chagas disease hospital database: a clinical and epidemiological patient profile. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 6, p. 831-835, NOV-DEC 2018. (15/24595-9)

TAMARINDO, GUILHERME H.; RIBEIRO, DANIELE L.; GOBBO, MARINA G.; GUERRA, LUIZ H. A.; RAHAL, PAULA; TABOGA, SEBASTIAO R.; GADELHA, FERNANDA R.; GOES, REJANE M.. Melatonin and Docosahexaenoic Acid Decrease Proliferation of PNT1A Prostate Benign Cells via Modulation of Mitochondrial Bioenergetics and ROS Production. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2019. (15/24595-9, 13/16368-7, 15/13371-2)

PARRA, LIZBETH L. L.; BERTONHA, ARIANE F.; SEVERO, IVAN R. M.; AGUIAR, ANNA C. C.; DE SOUZA, GUILHERME E.; OLIVA, GLAUCIUS; GUIDO, RAFAEL V. C.; GRAZZIA, NATHALIA; COSTA, TABATA R.; MIGUEL, DANILO C.; et al. Isolation, Derivative Synthesis, and Structure-Activity Relationships of Antiparasitic Bromopyrrole Alkaloids from the Marine Sponge Tedania brasiliensis. Journal of Natural Products, v. 81, n. 1, p. 188-202, JAN 2018. (15/24595-9, 13/07600-3, 14/21129-4, 13/50228-8)

NAKAMURA, ISABELLA BAGNI; MIGUEL, DANILO CICCONE; BRUSCATO, ANDRESSA; PEREIRA, MARIANE BARROSO; CAMPIOLO, DIMAS; DE ALMEIDA, EROS ANTONIO; PELOSO, EDUARDO DE FIGUEIREDO; GADELHA, FERNANDA RAMOS. Biological characterization of Trypanosoma cruzi epimastigotes derived from trypomastigotes isolated from Brazilian chagasic patients. CURRENT RESEARCH IN MICROBIAL SCIENCES, v. 3, p. 7-pg., 2022-01-01. (16/09112-4, 15/24595-9)

TAMARINDO, GUILHERME H.; RIBEIRO, DANIELE L.; GOBBO, MARINA G.; GUERRA, LUIZ H. A.; RAHAL, PAULA; TABOGA, SEBASTIAO R.; GADELHA, FERNANDA R.; GOES, REJANE M.. Melatonin and Docosahexaenoic Acid Decrease Proliferation of PNT1A Prostate Benign Cells via Modulation of Mitochondrial Bioenergetics and ROS Production. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2019, p. 15-pg., 2019-01-01. (15/13371-2, 13/16368-7, 15/24595-9)