ROLE OF ISCHEMIA IN THE PATHOGENESIS OF ASCENDING AORTA ANEURYSMS AND DISSECTIONS

• Grant number: 15/24077-8
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2016
• End date: February 28, 2018
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Paulo Sampaio Gutierrez
• Grantee: Paulo Sampaio Gutierrez
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

In ascending aorta aneurysms (AscAA) there is an enlargement of the whole vessel, while aortic dissections are characterized by the cleavage of the wall into two sheets at the external half. Both show similar alterations: mucoid pools and fine elastic fragmentation at the medial layer, without significant inflammation; smooth muscle cell apoptosis was also detected. The objective of the present project is to evaluate a potential role of ischemia in these diseases, through three approaches: 1) to verify the incidence of ischemic band necrosis and vasa vasorum alterations in AscAA; 2) analysis of mitochondria, that have a role in apoptosis; 3) evaluation of the expression of the hypoxia-inducible factor (HIFalpha1) and connexin-43 (altered in cardiac muscle cells in ischemia). Methods: 1) retrospective analysis of the last 1000 aortic surgical specimens of patients with AscAA withour rupture; 2) analysis by transmission electronic microscopy of 10 aortic specimens with acute dissection, and the same number with aneurysms, and, as controls, without such diseases, with and without systemic arterial hypertension, counting the number of mitochondria per area unit and the area fraction that they occupy; 3) evaluation of the expression of connexin-43 and HIFalpha1 by immunohistochemistry in samples corresponding to the same groups of the last topic, quantifying the positive percentage of area of the medial layer by area unit using a image analysis system coupled to a microscope. Statistical analysis: groups will be compared using two-way ANOVA. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUTIERREZ, PAULO SAMPAIO; MARQUES PIUBELLI, MARIO LUIZ; NAAL, KALIL GEORGETTO; DIAS, RICARDO RIBEIRO; BORGES, LUCIANO FIGUEIREDO. Orignal Mitochondria in aneurysms and dissections of the human ascending aorta {*}. CARDIOVASCULAR PATHOLOGY, v. 47, JUL-AUG 2020. (15/24077-8)

GUTIERREZ, PAULO SAMPAIO; MARQUES PIUBELLI, MARIO LUIZ; NAAL, KALIL GEORGETTO; DIAS, RICARDO RIBEIRO; BORGES, LUCIANO FIGUEIREDO. Orignal Mitochondria in aneurysms and dissections of the human ascending aorta *. CARDIOVASCULAR PATHOLOGY, v. 47, p. 5-pg., 2020-07-01. (15/24077-8)