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Multi-user equipment: acquisition of Amnis image stream to flow cytometry with image

Grant number: 16/06027-6
Support type:Multi-user Equipment Program
Duration: July 01, 2016 - June 30, 2023
Field of knowledge:Biological Sciences - Immunology - Cellular Immunology
Principal Investigator:Ana Marisa Chudzinski-Tavassi
Grantee:Ana Marisa Chudzinski-Tavassi
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival, AP.PCPE
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page:http://www.butantan.gov.br/pesquisa/equipamentos-multiusuarios
Tipo de equipamento:Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
Fabricante: Merck Milipore
Modelo: Amnis Image Stream

Abstract

The equipment will be used for the discovery of new molecular targets involved in inflammatory diseases, using animal venoms and toxins as tools for the study of these diseases. This equipment allows fluorescence intensity quantification in suspension cells, as any other flow cytometer. However, differently from other flow cytometers, it provides images of cells analyzed, since it is equipped with fluorescence microscope objectives. Thus, Amnis Image Stream is the only equipment available on the market able to study microparticles inside suspension cells and is essential for the development of this project, especially in experiments of drug delivery, besides other applications of conventional flow cytometry, such a as immunophenotyping, cell cycle analysis, cell viability, cell proliferation, phagocytosis, among others. (AU)