Corticotomy effect on orthodontic tooth movement. Histomorphometric and immunohistochemical study in rats.

• Grant number: 15/26523-5
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2016
• End date: July 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Maria Aparecida Neves Jardini
• Grantee: Maria Aparecida Neves Jardini
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The evolution of orthodontics brought an increasing number of adult patients seeking for orthodontic treatment, however adult patients are mainly concerned with aesthetics (the type of device) and the extent of treatment. A increasingly number of new techniques are being evaluated to diminish the treatment period to mention a few of these: the self-ligating brackets systems; memory ortho wires; mechanical or physical stimulation of alveolar bone, such as the use of direct electrical wire or magnetics, surgical intervention or administration of local or systemic drugs. Among those many surgical interventions, it is important to mention the corticotomy technique (CAS) or intentional injury of the cortical bone, which is limited to the cortical portion of the alveolar bone with minimal penetration into medullar bone and are indicated to accelerate the corrective orthodontic treatment as a whole. This study will compare two corticotomy protocols in an experimental model in mice and verify whether the increase in tooth movement rate is related to the different degrees of extension of the surgical procedure. For the experiment 48 Wistar rats will be used (Rattus norvegicus albinos) were randomly divided into 3 groups: Group 1 which received orthodontic movement and invasive corticotomy surgery (G1),Group 2 which received orthodontic movement and a less invasive corticotomy surgery (G2) and Group3 which received orthodontic movement only (G3). The sacrifices occur at 7 and 14 days after the procedure. For the analyzes results, the macroscopic measurements of the dental movement area will be performed, digital radiography, microCT (computed micro tomography), histomorphometric analysis in conventional microscopy and immunohistochemical reactions to identify and locate the factors involved in osteoclastogenesis (RANK, RANKL and OPG) . The final data will be submitted to an appropriate statistical analysis with 5% significance level. The descriptive statistics will involve the calculation of means and standard deviation, while inferential statistics will be performed by analysis of variance (ANOVA) and Tukey's test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARCELO LELIS ZUPPARDO; MILTON SANTAMARIA JR; CAMILA LOPES FERREIRA; MARIÉLLEN LONGO; JONI AUGUSTO CIRELLI; MAURO PEDRINE SANTAMARIA; MARIA APARECIDA NEVES JARDINI. Effect of two corticotomy protocols on periodontal tissue and orthodontic movement. Journal of Applied Oral Science, v. 28, 2020-07-03. (16/17141-4, 15/26523-5)

ZUPPARDO, MARCELO LELIS; FERREIRA, CAMILA LOPES; DE MOURA, NICOLE BERTON; LONGO, MARIELLEN; SANTAMARIA, JR., MILTON; PEREIRA CASTRO LOPES, SERGIO LUCIO; SANTAMARIA, MAURO PEDRINE; NEVES JARDINI, MARIA APARECIDA. Macroscopic and radiographic aspects of orthodontic movement associated with corticotomy: animal study. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, v. 23, n. 1, p. 77-82, MAR 2019. (16/17141-4, 15/26523-5)