Analysis Of Molecular Biomarkers Associated To Chemoresistance Derived From Breast Cancer Stem Cells

• Grant number: 16/07822-4
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: February 28, 2019
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Sophie Françoise Mauricette Derchain
• Grantee: Sophie Françoise Mauricette Derchain
• Host Institution: Centro de Atenção Integral à Saúde da Mulher (CAISM). Hospital da Mulher Professor Doutor José Aristodemo Pinotti. Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Ander Matheu Fernandez ; Luís Otávio Zanatta Sarian ; Marcelo Lima Ribeiro ; Rodrigo Menezes Jales

Abstract

Despite advances in molecular knowledge, breast cancer is still the most commonly diagnosed type of cancer and inducing more deaths in women worldwide, mainly due to the metastatic process and resistance to treatment. The DNA damage response pathway (DDR) is activated in response to genotoxic damage, controlling the cell cycle arrest or activating DNA repair. Studies showed that cancer stem cells (CSCs) can promote chemoresistance through DDR. Furthermore, it is known that the epithelial-mesenchymal transition (EMT) can generate cells with stem cells characteristics and therefore regulate the chemoresistance process. The exosomes are microvesicles comprised of RNAs, proteins and miRNAs that can be released by various cell types including tumor cells and CSCs. The exosomes allows the contents transfer cell to cell. Determine the reasons why a patient does not respond to chemotherapy, and thus be able to guide a most appropriate therapy for each patient remains formidable challenge in modern medicine. Fortunately, the genetic content present in circulating blood can provide clues and help change this scenario. We intend to evaluate the possible transfer of miRNAs that regulate chemoresistance among CSCs and tumor cells derived from breast of exosomes in order to understand the complexity of progression and therapy of breast cancer. Considering that the CSCs are able of forming a more aggressive tumor phenotype, with migration ability, metastasis, resistance to treatment and disease recurrence, as well as few studies to determine clearly the interaction of breast CSCs with its microenvironment, we intend to evaluate the possible exosome-mediated miRNAs transfer that regulate chemoresistance of breast CSCs and tumor cells, to help clarify the complexity of progression and therapy of breast cancer. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOSO, MARCELLA REGINA; SANTOS, JULIANA CARVALHO; RIBEIRO, MARCELO LIMA; RAMIRO TALARICO, MARIA CECILIA; VIANA, LAIS ROSA; MAURICETTE DERCHAIN, SOPHIE FRANCOISE. A Metabolomic Approach to Predict Breast Cancer Behavior and Chemotherapy Response. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 19, n. 2, FEB 2018. (16/07822-4)

DA SILVA, RODRIGO FERNANDES; CARDOZO, DANIELA MAIRA; LIBANIO RODRIGUES, GISELE OLINTO; DE SOUZA-ARAUJO, CAROLINE NATANIA; MIGITA, NATACHA AZUSSA; LUCCI DE ANGELO ANDRADE, LILIANA APARECIDA; DERCHAIN, SOPHIE; YUNES, JOSE ANDRES; GUIMARAES, FERNANDO. CAISMOV24, a new human low-grade serous ovarian carcinoma cell line. BMC CANCER, v. 17, NOV 13 2017. (11/14520-0, 16/07822-4, 14/07401-3, 12/12802-1)

CARDOSO, MARCELLA REGINA; SANTOS, JULIANA CARVALHO; RIBEIRO, MARCELO LIMA; RAMIRO TALARICO, MARIA CECILIA; VIANA, LAIS ROSA; MAURICETTE DERCHAIN, SOPHIE FRANCOISE. A Metabolomic Approach to Predict Breast Cancer Behavior and Chemotherapy Response. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 19, n. 2, p. 16-pg., 2018-02-01. (16/07822-4)

HOFFMANN, LUCAS GRECCO; SARIAN, LUIS OTAVIO; VASSALLO, JOSE; DE PAIVA SILVA, GEISILENE RUSSANO; BOTELHO RAMALHO, SUSANA OLIVEIRA; FERRACINI, AMANDA CANATO; ARAUJO, KARINA DA SILVA; JALES, RODRIGO MENEZES; FIGUEIRA, DEAYRA EMYLE; DERCHAIN, SOPHIE. Evaluation of PD-L1 and tumor infiltrating lymphocytes in paired pretreatment biopsies and post neoadjuvant chemotherapy surgical specimens of breast carcinoma. SCIENTIFIC REPORTS, v. 11, n. 1, NOV 18 2021. (16/07822-4)

ROSINI SILVA, ALEX AP.; CARDOSO, MARCELLA R.; REZENDE, LUCIANA MONTES; LIN, JOHN Q.; GUIMARAES, FERNANDO; PAIVA SILVA, GEISILENE R.; MURGU, MICHAEL; PRIOLLI, DENISE GONCALVES; EBERLIN, MARCOS N.; TATA, ALESSANDRA; et al. Multiplatform Investigation of Plasma and Tissue Lipid Signatures of Breast Cancer Using Mass Spectrometry Tools. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 10, MAY 2020. (16/07822-4, 19/04314-6)

BARRETA, AMILCAR; SARIAN, LUIS; FERRACINI, AMANDA CANATO; ELOY, LARISSA; CARVALHO BRITO, ANGELO BORSARELLI; ANDRADE, LILIANA DE ANGELO; DERCHAIN, SOPHIE. Endometriosis-Associated Ovarian Cancer Population Characteristics and Prognosis. International Journal of Gynecological Cancer, v. 28, n. 7, p. 1251-1257, SEP 2018. (12/15059-8, 16/07822-4)

BARRETA, AMILCAR; SARIAN, LUIS OTAVIO; FERRACINI, AMANDA CANATO; ELOY COSTA, LARISSA BASTOS; MAZZOLA, PRISCILA GAVA; ANDRADE, LILIANA DE ANGELO; DERCHAIN, SOPHIE. Immunohistochemistry expression of targeted therapies biomarkers in ovarian clear cell and endometrioid carcinomas (type I) and endometriosis. HUMAN PATHOLOGY, v. 85, p. 72-81, MAR 2019. (12/15059-8, 16/07822-4)

SANTOS, JULIANA CARVALHO; LIMA, NATALIA DA SILVA; SARIAN, LUIS OTAVIO; MATHEU, ANDER; RIBEIRO, MARCELO LIMA; MAURICETTE DERCHAIN, SOPHIE FRANCOISE. Exosome-mediated breast cancer chemoresistance via miR-155 transfer. SCIENTIFIC REPORTS, v. 8, JAN 16 2018. (15/25056-4, 16/07822-4)