| Grant number: | 16/05993-6 |
| Support Opportunities: | Regular Research Grants |
| Start date: | September 01, 2016 |
| End date: | August 31, 2018 |
| Field of knowledge: | Health Sciences - Medicine - Maternal and Child Health |
| Principal Investigator: | Ricardo Barini |
| Grantee: | Ricardo Barini |
| Host Institution: | Centro de Atenção Integral à Saúde da Mulher (CAISM). Hospital da Mulher Professor Doutor José Aristodemo Pinotti. Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Associated researchers: | Fernando Guimarães ; Juliana Karina Ruiz Heinrich Muçouçah |
Abstract
Background: Fetal-maternal hemorrhage (HFM) occurs in small quantities throughout pregnancy, increasing progressively until the later stages of pregnancy. If there is a significant difference in antigenicity between the fetus and the mother, awareness can be triggered in the mother's immune system. When undetected, silent hemorrhage can lead to consequences that jeopardize both the current pregnancy as future pregnancies. Detection of fetal red cells in maternal circulation is essential in managing patients treated with immunoglobulin anti D. Its use in prophylaxis is almost universal, but with regional dosage ranges. Thus, the sensitivity and specificity become decisive factors in the detection of HFM to therapeutic efficacy and subsequent clinical results. Objective: To compare the accuracy of flow cytometry for the detection of fetal red cells in maternal blood circulation using immunological techniques for identification of different cell populations to an automatic analyzer for blood oximetry through the differentiation of cell populations. Subjects and Methods: an experimental test study will be conducted with 24 samples of approximately 10 ml of adult blood in males among the companions of patients treated at Gestational Loss Clinic (CAISM) or at the time of blood donation at the Blood Center of UNICAMP. We'll use up to 10 ml of cord blood collected moments after birth at the Hospital of Prof. Women Dr. Jose Aristodemo Pinotti CAISM / UNICAMP. Fetal samples will be subjected to dilutions in adult blood to identify amounts of fetal and adult red blood cells present, tested at each analysis unit. Data analysis: The test used to calculate the sample size was tested for the difference between two proportions (Z test with normal approximation) with bilateral hypothesis, significance level (±) equal to 5% and null hypothesis of no difference between ratios. (AU)
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