Vegetative structure of the achlorophyllous orchid Pogoniopsis schenckii (Vanilloideae) and its interaction with mycorrhizal fungi

• Grant number: 15/26479-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2016
• End date: October 31, 2019
• Field of knowledge: Biological Sciences - Botany - Plant Morphology
• Principal Investigator: Juliana Lischka Sampaio Mayer
• Grantee: Juliana Lischka Sampaio Mayer
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Carlos Eduardo Pereira Nunes ; Maria Leticia Bonatelli ; Rafael Silva Oliveira ; Sara Adrian Lopez de Andrade

Abstract

Pogoniopsis, a little-known genus of the Orchidaceae, consists of two achlorophyllous species, with obligatory myco-heterotrophic metabolisms, it has a pale yellow color and develop in the organic matter in dense tropical forests. Myco-heterotrophic (MH) plants depend entirely on the association with mycorrhizal fungi throughout their lives cycle. This association is required for obtaining carbohydrates and other nutrients such as N and P, for example. The phylogenetic relationships of Pogoniopsis remain uncertain to date and studies of this MH species may be important for elucidating the evolutionary history of Vanilloideae and it can also contribute to gain understanding the evolution and specialization of these plants. The objectives of this project are to understand the organization of the underground vegetative structure and observe the pattern of fungal colonization associated with this kind MH through analysis in optical microscopy and scanning and transmission electron microscopy. The fungi present in these subterranean orchid organs will be isolated and identified. In addition, it will be assessed whether the isolated fungi will be capable of forming mycorrhizal association with the embryo in the mature seed and promote protocorms development. In addition, it will be analyzed the isotopic composition of the plant biomass, the carbon (´13C) and nitrogen (´15N) ratios, in order to assist the interpretation of the MH nature of this species indicating a possible specificity to certain fungal hosts and the origin of the ultimate C and N sources for this rare orchid species. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SISTI, LAIS SOEMIS; ALINE FLORES-BORGES, DENISELE NEUZA; LOPEZ DE ANDRADE, SARA ADRIAN; KOEHLER, SAMANTHA; BONATELLI, MARIA LETICIA; SAMPAIO MAYER, JULIANA LISCHKA. The Role of Non-Mycorrhizal Fungi in Germination of the Mycoheterotrophic Orchid Pogoniopsis schenckii Cogn.. FRONTIERS IN PLANT SCIENCE, v. 10, NOV 29 2019. (15/26479-6)

SISTI, LAIS SOEMIS; PENA-PASSOS, MATHEUS; MAYER, JULIANA LISHCKA SAMPAIO. Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v. N/A, n. 195, p. 23-pg., 2023-05-01. (15/26479-6)

KLIMPERT, NATHANIEL J. J.; MAYER, JULIANA LISCHKA SAMPAIO; SARZI, DEISE SCHRODER; PROSDOCIMI, FRANCISCO; PINHEIRO, FABIO; GRAHAM, SEAN W. W.. Phylogenomics and plastome evolution of a Brazilian mycoheterotrophic orchid, Pogoniopsis schenckii. AMERICAN JOURNAL OF BOTANY, v. 109, n. 12, p. 21-pg., 2022-11-18. (15/26479-6, 16/22785-8)

GALBIATTI, MARIA ISABEL; CASSOLA, FABIO; MESQUITA, AMANDA TEIXEIRA; PINHEIRO, GUILHERME PEREZ; SAMPAIO MAYER, JULIANA LISCHKA; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. Plectranthus neochilus Schltr.: Anatomic and cytogenetic analyses and chemical characterization of its essential oil. SOUTH AFRICAN JOURNAL OF BOTANY, v. 143, p. 97-106, DEC 2021. (15/26479-6)

PENA-PASSOS, MATHEUS; SISTI, LAIS SOEMIS; SAMPAIO MAYER, JULIANA LISCHKA. Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v. N/A, n. 183, p. 30-pg., 2022-05-01. (15/26479-6)

ALVES, MARIANA FERREIRA; PINHEIRO, FABIO; NIEDZWIEDZKI, MARTA PINHEIRO; SAMPAIO MAYER, JULIANA LISCHKA. First Record of Ategmic Ovules in Orchidaceae Offers New Insights Into Mycoheterotrophic Plants. FRONTIERS IN PLANT SCIENCE, v. 10, NOV 29 2019. (15/26479-6)

CASSOLA, FABIO; PEREIRA NUNES, CARLOS EDUARDO; LUSA, MAKELI GARIBOTTI; GARCIA, VERA LUCIA; SAMPAIO MAYER, JULIANA LISCHKA. Deep in the Jelly: Histochemical and Functional Aspects of Mucilage-Secreting Floral Colleters in the Orchids Elleanthus brasiliensis and E. crinipes. FRONTIERS IN PLANT SCIENCE, v. 10, APR 24 2019. (03/12595-7, 15/26479-6)

ALVES, MARIANA FERREIRA; PINHEIRO, FABIO; PEREIRA NUNES, CARLOS EDUARDO; PROSDOCIMI, FRANCISCO; SARZI, DEISE SCHRODER; FURTADO, CAROLINA; SAMPAIO MAYER, JULIANA LISCHKA. Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn.. BMC PLANT BIOLOGY, v. 21, n. 1, JUL 12 2021. (16/22785-8, 15/26479-6)