Potential of Filamentous Fungi for Production of Exo Hydrolases with Biotechnological Interest: Influence of Fermentative Process on Morphology, Cytoskeleton and Secretion of ²-D-fructofuranosidases, Phytases and Tannases

• Grant number: 16/11311-5
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2016
• End date: October 31, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luis Henrique Souza Guimaraes
• Grantee: Luis Henrique Souza Guimaraes
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The world global market of industrial enzymes moves billions of dollars per year, with an estimative of US$ 6.2 billions of dollars for 2020. Under this context, the demand by enzymes with new properties is growing each year. On the other hand, the obtainment of enzymes has used the same sources, without novelties. The microorganisms are the most important and attractive sources of enzymes, especially the filamentous fungi. Considering this aspect there is a reduced numbers of fungal strains able to produce these biomolecules considering the microbial diversity, which still stay poorly explored. The production of enzymes by filamentous fungi has been performed using different fermentative process as Solid-State Fermentation (SSF) and Submerged Fermentation (SbmF). In the last years, a new alternative technology was proposed for production of enzymes, the Biofilm Fermentation (BF), which associates advantages from SSF and SbmF. Them, main objective of this project is to study the production of exo-hydrolases with biotechnological potential (²-D-fructofuranosidases, phytases and tannases) by filamentous fungi under SbmF and BF, considering especially the morphology patterns. It is known that the fungal morphology under both fermentation processes is different, what is very relevant considering that the fungal morphology has direct influence on the enzyme secretion. However, a depth study considering this aspect focused on the cytoskeleton proteins (actin and microtubules) was not conducted until this moment. To control the fungal morphology is an important way to improve the production of enzymes with biotechnological application. Additionally, the enzymes obtained will be purified and biochemically characterized. Then, this project can be characterized as basic and applied research for different knowledge areas (biological diversity, biochemistry, microbiology and biotechnology). (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FARIAS CAVALCANTI, RAYZA MORGANNA; LOMBARDI MARTINEZ, MARCELO LUIS; OLIVEIRA, WANDERLEY PEREIRA; SOUZA GUIMARAES, LUIS HENRIQUE. Stabilization and application of spray-dried tannase from Aspergillus fumigatus CAS21 in the presence of different carriers. 3 BIOTECH, v. 10, n. 4, MAR 25 2020. (16/11311-5)

ARACRI, FERNANDA MANSANO; FARIAS CAVALCANTI, RAYZA MORGANNA; SOUZA GUIMARAES, LUIS HENRIQUE. Extracellular Tannase from Aspergillus ochraceus: Influence of the Culture Conditions on Biofilm Formation, Enzyme Production, and Application. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 29, n. 11, p. 1749-1759, NOV 2019. (16/11311-5)

FARIAS CAVALCANTI, RAYZA MORGANNA; MAESTRELLO, CHADIA CHAHUD; SOUZA GUIMARAES, LUIS HENRIQUE. Immobilization of the Tannase From Aspergillus fumigatus CAS21: Screening the Best Derivative for the Treatment of Tannery Effluent Using a Packed Bed Reactor. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 9, NOV 3 2021. (16/11311-5)

FARIAS CAVALCANTI, RAYZA MORGANNA; JORGE, JOAO ATILIO; SOUZA GUIMARAES, LUIS HENRIQUE. Characterization of Aspergillus fumigatus CAS-21 tannase with potential for propyl gallate synthesis and treatment of tannery effluent from leather industry. 3 BIOTECH, v. 8, n. 6, p. 11-pg., 2018-05-25. (16/11311-5)

FARIAS CAVALCANTI, RAYZA MORGANNA; LOMBARDI MARTINEZ, MARCELO LUIS; OLIVEIRA, WANDERLEY PEREIRA; SOUZA GUIMARAES, LUIS HENRIQUE. Stabilization and application of spray-dried tannase from Aspergillus fumigatus CAS21 in the presence of different carriers. 3 BIOTECH, v. 10, n. 4, p. 14-pg., 2020-03-25. (16/11311-5)

FARIAS CAVALCANTI, RAYZA MORGANNA; JORGE, JOAO ATILIO; SOUZA GUIMARAES, LUIS HENRIQUE. Characterization of Aspergillus fumigatus CAS-21 tannase with potential for propyl gallate synthesis and treatment of tannery effluent from leather industry. 3 BIOTECH, v. 8, n. 6, MAY 25 2018. (16/11311-5)

TODERO, LARISSA MIDIANE; VARGAS RECHIA, CAREM GLEDES; SOUZA GUIMARAES, LUIS HENRIQUE. Production of short-chain fructooligosaccharides (scFOS) using extracellular beta-D-fructofuranosidase produced by Aspergillus thermomutatus. JOURNAL OF FOOD BIOCHEMISTRY, v. 43, n. 8, AUG 2019. (16/11311-5)