Liofilization of biological tissue: research from the point of view of development of biomaterials

• Grant number: 04/09566-8
• Support Opportunities: Research Projects - Thematic Grants
• Start date: April 01, 2005
• End date: September 30, 2010
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Ronaldo Nogueira de Moraes Pitombo
• Grantee: Ronaldo Nogueira de Moraes Pitombo
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Marina Junko Shiotsu Maizato ; Marisa Masumi Beppu ; Olga Zazuco Higa

Abstract

The liofilization process when applied to human or animal biological tissue, allows these to be preserved dry, facilitating their handling and storage, permitting gamma ray sterilization or other means which guarantee an effective sterilization of the material, especially the elimination of slow viruses, for subsequent use as medical-hospital products (graft, prostheses, etc.). The liofilization of biological tissues does not significantly alter the mechanical characteristics of the product and its reconstitution is simple. In the study of liofilization there is an interesting demonstration of the subtleties of the relationships of water with the biological materials. It is a process of non-equilibrium, which when well conducted, is under kinetic control and involves metastable vitreous states. Understanding it is linked to the phase transitions, as well as, rheological and structural phenomena. Although it is a sophisticated technology, it is far from maturity, which justifies an in-depth investigation of each product. Therefore, for its development and use, aiming for final application of the process in cutting edge technological products, liofilization requires a multi-disciplinary approach, bringing together researchers from the diverse biological, biochemical, exact and medical areas of knowledge, which integrated in the same objective can reveal all the objectives proposed in the present thematic project. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAIZATO‚ M.J.S.; PIRES‚ M.D.; CANZIAN‚ M.; HIGA‚ O.Z.; PITOMBO‚ R.N.M.; LEIRNER‚ A.A.. Histological evaluation of biocompatibility of lyophilized bovine pericardium implanted subcutaneously in rats. ARTIFICIAL ORGANS, v. 32, n. 4, p. 268-271, 2008. (04/09566-8)

RODAS‚ A.C.D.; MAIZATO‚ M.J.S.; LEIRNER‚ A.A.; PITOMBO‚ R.N.M.; POLAKIEWICZ‚ B.; BEPPU‚ M.M.; HIGA‚ O.Z.. Cytotoxicity and genotoxicity of bovine pericardium preserved in glycerol. ARTIFICIAL ORGANS, v. 32, n. 4, p. 272-276, 2008. (04/09566-8)

MAIZATO, MARINA J. S.; TANIGUCHI, FABIO P.; AMBAR, RAFAEL F.; PITOMBO, RONALDO N. M.; LEIRNER, ADOLFO A.; CESTARI, IDAGENE A.; STOLF, NOEDIR A. G.. Behavior of Lyophilized Biological Valves in a Chronic Animal Model. ARTIFICIAL ORGANS, v. 37, n. 11, p. 958-964, NOV 2013. (04/09566-8)

MAIZATO, MARINA J. S.; HAYASHIDA, SERGIO A.; TANIGUCHI, FABIO P.; HIGA, OLGA Z.; PITOMBO, RONALDO N. M.; CESTARI, IDAGENE A.; LEIRNER, ADOLFO A.; STOLF, NOEDIR A. G.. In Vitro and In Vivo Evaluation of Lyophilized Bioprosthetic Valve. ARTIFICIAL ORGANS, v. 35, n. 5, p. 490-496, MAY 2011. (04/09566-8)

Filed patent(s) as a result of this research project