Comparative analysis of reticulin and collagen fibers' distribution in patients with sarcoidosis and tuberculoid leprosy

• Grant number: 16/22927-7
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2017
• End date: February 28, 2018
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Maria Letícia Cintra
• Grantee: Maria Letícia Cintra
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The differential diagnosis between cutaneous sarcoidosis and tuberculoid leprosy ( MHT ) is not easily achieved because the clinical and histological findings may be similar. The texture analysis of collagen and reticulin fibers and the immunophenotypic profile of inflammatory cells in skin samples could help to distinguish the two processes and add elements to the understanding of the pathogenesis of both diseases. Skin samples from patients with MHT and with sarcoidosis, matched for age and sex, will be studied. Clinical data of each patient will be obtained from HC - UNICAMP medical files. Digital images from reticulin will be acquired in a blinded way and analyzed through ImageJ software. To study collagen, images will be acquired at the National Institute of Applied Photonics for Cell Biology (INFABIC), in a multiphoton microscope, with the technique of second harmonic generation (SHG), using laserTi sapphire in 940nm in immersion oil and 40x, in routine staining sections. The results will be tested under the following statistical methods: Pearson correlation, ANOVA and Student t test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

UTINO, FABIANE LEONEL; DAMIANI, GISLAINE VIEIRA; GARCIA, MARINA; BENETTI SOARES, TANIA CRISTINA; STELINI, RAFAEL FANTELLI; NEVES FERREIRA VELHO, PAULO EDUARDO; DE SOUZA, ELEMIR MACEDO; CINTRA, MARIA LETICIA. Histomorphometric approach to differentiate skin lesions of tuberculoid leprosy from sarcoidosis. JOURNAL OF CUTANEOUS PATHOLOGY, v. 45, n. 2, p. 111-117, FEB 2018. (16/22927-7)

UTINO, FABIANE LEONEL; GARCIA, MARINA; NEVES FERREIRA VELHO, PAULO EDUARDO; ELOY DA COSTA FRANCA, ANDREA FERNANDES; STELINI, RAFAEL FANTELLI; PELEGATI, VITOR BIANCHIN; CESAR, CARLOS LENZ; DE SOUZA, ELEMIR MACEDO; CINTRA, MARIA LETICIA; DAMIANI, GISLAINE VIEIRA. Second-harmonic generation imaging analysis can help distinguish sarcoidosis from tuberculoid leprosy. Journal of Biomedical Optics, v. 23, n. 12, DEC 2018. (17/17149-8, 16/22927-7, 14/50938-8, 11/51959-0)