Use of sperm attributes to create a predictive model for in vitro embryo production

• Grant number: 16/15147-5
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2017
• End date: February 28, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Mayra Elena Ortiz D' Avila Assumpcao
• Grantee: Mayra Elena Ortiz D' Avila Assumpcao
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Adriano Felipe Perez Siqueira ; Marcilio Nichi

Abstract

In vitro production (IVP) of bovine embryos is an important technology of animal reproduction. This technology is applied on research and livestock, with economic highlights. Despite its applicability, the efficiency of IVP is considered limited when compared to the in vivo process. The effect of sperm in embryonic development has been described but it is not clear yet which attributes directly impact the blastocyst rate. The aim of this study is to identify which attributes contribute more to compose the predictive model of blastocyst production. Therefore, there will be a prospective analysis of a large number of semen samples, combining different bulls and batches (n = 50). For each sample will be collect flow cytometry evaluation (plasma membrane and acrosome integrity, mitochondrial membrane potential and chromatin integrity) and computerized motility (motility parameters and sperm subpopulations). The same samples will be used for in vitro embryos production, which will be evaluate by cleavage and blastocyst rates, and differential cell counts to build the predictive model of in vitro embryo production. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREZ SIQUEIRA, ADRIANO FELIPE; DE CASTRO, LETICIA SIGNORI; DE ASSIS, PATRICIA MONKEN; BICUDO, LUANA DE CASSIA; MENDES, CAMILLA MOTA; NICHI, MARCILLIO; VISINTIN, JOSE ANTONIO; ORTIZ D'AVILA ASSUMPCAO, MAYRA ELENA. Sperm traits on in vitro production (IVP) of bovine embryos: Too much of anything is good for nothing. PLoS One, v. 13, n. 7, JUL 10 2018. (13/07940-9, 16/15147-5)

DE ALMEIDA, TAMIE GUIBU; MINGOTI, RODOLFO DANIEL; DE CASTRO, LETICIA SIGNORI; PEREZ SIQUEIRA, ADRIANO FELIPE; DOS SANTOS HAMILTON, THAIS ROSE; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; ALVES, MAYRA FERNANDA; BASSO, ANDREA CRISTINA; MILAZZOTTO, MARCELLA PECORA; et al. Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile. Theriogenology, v. 178, p. 30-39, JAN 15 2022. (16/15147-5, 12/50533-2)