Immobilization of Amano lipase from Pseudomonas fluorescens on silk fibroin spheres: an alternative protocol for the enantioselective synthesis of halohydrins

• Grant number: 17/05795-2
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: May 01, 2017
• End date: October 31, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Andre Luiz Meleiro Porto
• Grantee: Andre Luiz Meleiro Porto
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

The search for a new, efficient, cheaper and sustainable matrix for lipase immobilization is a growing area inbiotechnology. Amano lipase from Pseudomonas fluorescens was immobilized on silk fibroin spheres andused in the enzymatic kinetic resolution of halohydrins, to obtain optically active epoxides (up to 99% ee),important precursors in the synthesis of derivative antifungal azoles. This paper reinforces the versatility ofsilk fibroin as a support for heterogeneous catalysts. (AU)