DENTAL PULP STEM CELLS BY BLOOD CATHETERIZATION FOR NERVE REGENERATION IN RABBITS

• Grant number: 16/24813-9
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2017
• End date: March 31, 2018
• Field of knowledge: Health Sciences - Medicine - Medical Radiology
• Principal Investigator: Heloísa Juliana Zabeu Rossi Costa
• Grantee: Heloísa Juliana Zabeu Rossi Costa
• Host Institution: Fundação Faculdade de Medicina da Universidade de São Paulo (FUNDFMUSP). São Paulo , SP, Brazil

Abstract

In cases of severe facial paralysis that affect the labirintic portion of the facial nerve, the treatment is fossa media decompression, an extremelly invasive procedure that carries 30% of main complications and has controversial results. We seek a less invasive and more efficient therapeutic for this disease.Our study is a preclinical trial for treatment of inflamatory facial nerve paralysis (without nerve section) with pulp derived stem cells by intrarterial catheterism.We are going to make an injury by compression of the main extratemporal root of the facial nerve in 30 New Zealand rabbits,male, with 200-300kg. These animals will be divided in two groups: control (A) and treated (B). Group A will receive saline solution and group B, derived dental pulp stem cells by catheterism. After four weeks, animals will be sacrificed for histological analysis. Functional analysis (compound muscle action potential) will be made twice: two weeks after catheterism and just before the sacrifice. (AU)