Analysis of the effect of human HSP27 overexpression on the longevity of Saccharomyces cerevisiae

• Grant number: 17/09938-2
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2017
• End date: July 31, 2019
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: José Ribamar dos Santos Ferreira Júnior
• Grantee: José Ribamar dos Santos Ferreira Júnior
• Host Institution: Escola de Artes, Ciências e Humanidades (EACH). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The aging population is constantly growing, and accompanies a higher incidence of chronic and neurodegenerative diseases such as Alzheimer's, Parkinson's and Huntington. These diseases are caused by the accumulation of unfolded proteins, and studies have indicated that the components of the ubiquitin-proteasome system are a potential therapeutic target for diseases related to the senile process of aging. The overexpression of Hsp27 chaperone can increase the catalytic activity of the proteasome and degradation of ubiquitinated proteins. This project aims to analyze the effect of overexpression of Hsp27 human, under the control of eleven TEF mutant promoters, in the longevity of S. cerevisiae. The assays that will be performed involve measuring the chronological and replicative life span, thermotolerance, resistance to oxidative stress and determination of proteasomal activity. Knowledge of how Hsp27 acts on proteasome to control longevity is essential for the future development of therapies that could control the gene expression of the protein and modulate the phenotypes observed in human senescence. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MONTANARI FUKUDA, ANA PAULA; CAMANDONA, VITTORIA DE LIMA; MENDONCA FRANCISCO, KELLITON JOSE; RIOS-ANJOS, RAFAELA MARIA; DO LAGO, CLAUDIMIR LUCIO; FERREIRA-JUNIOR, JOSE RIBAMAR. Simulated microgravity accelerates aging in Saccharomyces cerevisiae. LIFE SCIENCES IN SPACE RESEARCH, v. 28, p. 32-40, FEB 2021. (17/09938-2)

CAMANDONA, VITTORIA DE LIMA; RIOS-ANJOS, RAFAELA MARIA; ALEGRIA, THIAGO GERONIMO PIRES; PEREIRA, FABIO; BICEV, RENATA NAPORANO; DA CUNHA, FERNANDA MARQUES; DIGIAMPIETRI, LUCIANO ANTONIO; DE BARROS, MARIO HENRIQUE; NETTO, LUIS EDUARDO SOARES; FERREIRA-JUNIOR, JOSE RIBAMAR. Expression of human HSP27 in yeast extends replicative lifespan and uncovers a hormetic response. BIOGERONTOLOGY, v. 21, n. 5, MAR 2020. (17/09938-2, 13/07937-8, 16/11724-8)