Determination of heavy metals in traditional herbal medicines by alternative analytical techniques employing green chemistry principles

• Grant number: 16/25256-6
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2017
• End date: December 31, 2019
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Leandro Augusto Calixto
• Grantee: Leandro Augusto Calixto
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: Bruno Lemos Batista ; Débora Cristina de Oliveira ; Felipe Rebello Lourenço ; Geórgia Christina Labuto Araújo ; Maria de Lourdes Leite de Moraes ; Marina Franco Maggi Tavares

Abstract

The use of plants for medicinal purposes is widespread in the entire world. Their popularity is due to the ease of access, therapeutic effectiveness, relatively low cost and the belief that do not exhibit toxic effects. Herbal are basically divided into two classes: i) herbal medicines (HM) and herbal traditional products (HTP), evidence of safety and efficacy occurs by means of clinical studies for HM, and for HTP occurs by time of use based on technical-scientific literature. Without the need for medical prescription, HTP consumption occurs in the empirical way, based on self-medication, and in many cases without any information about the product origin. Poisonings reported in the literature associated with the presence of lead, mercury, arsenic and cadmium show the need for rigorous quality control analysis for HTP. However, the pharmacopoeial methods for heavy metal determination have been the same for more than one century, and these do not meet toxicological limits established by ICH Q3D. U.S. Pharmacopeial Convention (USP), aware of this need, has published a new chapter for metallic impurities analysis by inductively coupled plasma (ICP). Due to the high costs of acquisition and maintenance of ICPs, mainly for small manufacturers and compounding pharmacies, this project aims to develop alternative analytical methodologies using capillary electrophoresis (CE) associated with on-line pre-concentration techniques (stacking e sweeping) and off-line, employing green chemistry precepts, such as: microextraction techniques and cloud point extraction (CPE) for heavy metals determination in notified HTP marketed in Brazil. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATHEUS SANTIAGO DA SILVA; GREYCE KELLY STEINHORST CALIXTO; DÉBORA CRISTINA DE OLIVEIRA; FELIPE REBELLO LOURENÇO; LEANDRO AUGUSTO CALIXTO. Desirability Function in analytical method development for determination of glitazones and metabolites employing HF-LPME. Brazilian Journal of Pharmaceutical Sciences, v. 58, 2022-05-23. (16/25256-6)

DE FRANCA, MICHELLE LOREDO; SEPAROVIC, LUCIANA; JUNIOR, LUIZ SIDNEY LONGO; DE OLIVEIRA, DEBORA CRISTINA; LOURENCO, FELIPE REBELLO; CALIXTO, LEANDRO AUGUSTO. Determining uncertainty in a simple UV-Vis spectrometry method employing dimethyl carbonate as green solvent for lead determination in water. MEASUREMENT, v. 173, MAR 2021. (16/25256-6)

DA SILVA, FERNANDO ANTUNES; LOURENCO, FELIPE REBELLO; CALIXTO, LEANDRO AUGUSTO. Multiple Optimization for Extraction of Ethinylestradiol, Levonorgestrel, and Their Main Impurities using Mixture Design. JOURNAL OF ANALYTICAL CHEMISTRY, v. 77, n. 7, p. 9-pg., 2022-07-01. (16/25256-6)

DA SILVA, FERNANDO ANTUNES; LOURENCO, FELIPE REBELLO; CALIXTO, LEANDRO AUGUSTO. Development and optimization of stability-indicating method of ethinylestradiol, levonorgestrel, and their main impurities using quality by design approach. Journal of Pharmaceutical and Biomedical Analysis, v. 225, p. 14-pg., 2022-12-29. (16/25256-6)

GRECCO, GIULIA BRICK; ALBINI, KATHLEEN FIORAMONTE; LONGO JR, LUIZ SIDNEY; ANDREO, MARCIO ADRIANO; BATISTA, BRUNO LEMOS; LOURENCO, FELIPE REBELLO; CALIXTO, LEANDRO AUGUSTO. Application of experimental design for dispersive liquid-liquid microextraction optimization for metallic impurities determination in arnica infusion employing green solvents. Journal of the Iranian Chemical Society, v. N/A, p. 10-pg., 2022-10-08. (16/25256-6)

KLASSEN, ALINE; FERNANDES, RAFAEL FERREIRA; DE OLIVEIRA, DEBORA CRISTINA; DO NASCIMENTO, MARIA PATRICIA; BORGES, MARCELLA MATOS CORDEIRO; DE OLIVEIRA, MARCONE AUGUSTO LEAL; CALIXTO, LEANDRO AUGUSTO; BORGES, KEYLLER BASTOS. Short-End Injection Capillary Electrophoresis and Multivariate Analysis for Simultaneous Determination of Heavy Metals in Passiflora incarnata Tea. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, v. 19, n. 23, p. 10-pg., 2022-12-01. (16/25256-6)