Unveiling the association of Colletotrichum species with soybean anthracnose using histological, populational and genomic approaches

• Grant number: 17/09178-8
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2017
• End date: January 31, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Nelson Sidnei Massola Júnior
• Grantee: Nelson Sidnei Massola Júnior
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Maisa Ciampi Guillardi ; Pierre Gladieux ; Riccardo Baroncelli

Abstract

The large soybean cultivation in Brazil, mostly under monoculture and non-tillage system, affects the intensity of diseases. Amongst them, anthracnose is the most prominent, as soybeans are susceptible to infection at all stages of development. Frequent reports on the increase incidence in the northern and mid-western regions indicate that chemical control program for fungal diseases in soybean has not been effective against anthracnose. Up to 2007, only C. truncatum had been reported as the causal agent of the disease in Brazil; an additional association of C. cliviae was reported from 2012 on in Mato Grosso State. To better understand the association of pathogenic Colletotrichum species to soybean anthracnose, this proposal aims: a) developing a LAMP-based test to detect C. cliviae in asymptomatic soybean seeds and plant samples; b) elucidating the direction of transmission of C. cliviae and C. truncatum from soybean seeds and plants; c) testing in vitro sensibility of C. cliviae (and C. truncatum?) to fungicides; d) accessing the genetic structure of C. truncatum populations, based on SSR markers and multilocus sequencing; d) inferring the predominant reproductive mode in these populations; e) fine-scale investigating demographic processes, using SNP markers in a population genomic approach; f) examining the influence on the development of anthracnose symptoms by pathogenic C. cliviae and C. truncatum strains through inoculations on soybean plants; g) studying the histology of colonization of soybean plants by C. cliviae and C. truncatum strains; h) identifying differentially expressed genes by pathogenic C. cliviae and C. truncatum strains, based on RNAseq, in distinct soybean growth stages. Knowing the direction of C. cliviae and C. truncatum transmission could indicate if there is need for seed treatment with the evaluated fungicides, and a rapid LAMP test could diagnose C. cliviae in soybean seeds and plants. Based on efficient mechanisms of pathogen dissemination over long distances and the levels of recombination previously reported, we tested the hypotheses: (i) that C. truncatum populations are non-differentiated across soybean fields; (ii) that this pathogen is recombinant in structure (iii) that the current geographic distribution of C. truncatum reflects the historical expansion of soybean crops in Brazil, from South towards Central-West and North regions. The genomic approach might provide clues of what makes C. truncatum such a successful pathogen. Investigating the histology of soybean plants infected by C. cliviae and C. truncatum will enable us to better understand the strategy of cellular colonization selected by these distinct Colletotrichum species. Through the identification of differentially expressed genes between pathogenic C. cliviae and C. truncatum strains by comparison of transcriptome profiles expressed in each fungal species might indicate what biochemical pathways are recruited in each step of plant growth stage. A clear understanding of the factors responsible for infection and colonization of C. cliviae and C. truncatum is essential to develop long-term control measures to manage anthracnose. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOUFLEUR, THAIS R.; CIAMPI-GUILLARDI, MAISA; TIKAMI, ISIS; ROGERIO, FLAVIA; THON, MICHAEL R.; SUKNO, SERENELLA A.; MASSOLA JUNIOR, NELSON S.; BARONCELLI, RICCARDO. Soybean anthracnose caused by Colletotrichum species: Current status and future prospects. MOLECULAR PLANT PATHOLOGY, v. 22, n. 4, FEB 2021. (17/09178-8)

BOUFLEUR, THAIS R.; MASSOLA JUNIOR, NELSON S.; TIKAMI, ISIS; SUKNO, SERENELLA A.; THON, MICHAEL R.; BARONCELLI, RICCARDO. Identification and Comparison of Colletotrichum Secreted Effector Candidates Reveal Two Independent Lineages Pathogenic to Soybean. PATHOGENS, v. 10, n. 11, p. 16-pg., 2021-11-01. (17/09178-8)

ROGERIO, FLAVIA; BOUFLEUR, THAIS R.; CIAMPI-GUILLARDI, MAISA; SUKNO, SERENELLA A.; THON, MICHAEL R.; MASSOLA JUNIOR, NELSON SIDNEI; BARONCELLI, RICCARDO. Genome Sequence Resources of Colletotrichum truncatum, C. plurivorum, C. musicola, and C. sojae: Four Species Pathogenic to Soybean (Glycine max). PHYTOPATHOLOGY, v. 110, n. 9, p. 1497-1499, SEP 2020. (17/09178-8)

LINHARES DE CASTRO, RENATA REBELLATO; CIAMPI-GUILLARDI, MAISA; ROGERIO, FLAVIA; DA SILVA, CLAUDIO DIAS, JR.; BOUFLEUR, THAIS REGINA; MASSOLA, NELSON SIDNEI, JR.. Species diversity, resistance to MBC fungicides, and low sensitivity to azoxystrobin in field isolates of Colletotrichum spp. associated with soybean anthracnose in Mato Grosso and Goias States, Brazil. ROPICAL PLANT PATHOLOG, v. N/A, p. 12-pg., 2022-12-22. (17/09178-8, 21/01606-6)

BOUFLEUR, T. R.; CASTRO, R. R. L.; ROGERIO, F.; CIAMPI-GUILLARDI, M.; BARONCELLI, R.; MASSOLA JUNIOR, N. S.. First Report of Colletotrichum musicola Causing Soybean Anthracnose in Brazil. PLANT DISEASE, v. 104, n. 6, p. 1-pg., 2020-06-01. (17/09178-8)

ROGERIO, FLAVIA; GLADIEUX, PIERRE; MASSOLA, JR., NELSON SIDNEI; CIAMPI-GUILLARDI, MAISA. Multiple Introductions Without Admixture of Colletotrichum truncatum Associated with Soybean Anthracnose in Brazil. PHYTOPATHOLOGY, v. 109, n. 4, p. 681-689, APR 2019. (17/09178-8)

CAFA, GIOVANNI; BOUFLEUR, THAIS REGINA; LINHARES DE CASTRO, RENATA REBELLATO; MASSOLA JR, NELSON SIDNEI; BARONCELLI, RICCARDO. Genome Sequence Data of the Soybean Pathogen Stagonosporopsis vannaccii: A Resource for Studies on Didymellaceae Evolution. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 33, n. 8, p. 1022-1024, AUG 2020. (17/09178-8)

BOUFLEUR, THAIS R.; MASSOLA JR, NELSON S. S.; BECERRA, SIOLY; BARALDI, ELENA; BIBIANO, LILLIAN B. J.; SUKNO, SERENELLA A. A.; THON, MICHAEL R. R.; BARONCELLI, RICCARDO. Comparative transcriptomic provides novel insights into the soybean response to Colletotrichum truncatum infection. FRONTIERS IN PLANT SCIENCE, v. 13, p. 15-pg., 2022-11-25. (17/09178-8, 21/01606-6)

ROGERIO, FLAVIA; VAN OOSTERHOUT, COCK; CIAMPI-GUILLARDI, MAISA; CORRER, FERNANDO HENRIQUE; HOSAKA, GUILHERME KENICHI; CROS-ARTEIL, SANDRINE; RODRIGUES ALVES MARGARIDO, GABRIEL; MASSOLA JUNIOR, NELSON S.; GLADIEUX, PIERRE. Means, motive and opportunity for biological invasions: Genetic introgression in a fungal pathogen. Molecular Ecology, v. N/A, p. 15-pg., 2022-02-06. (17/09178-8)

TIKAMI, ISIS; BARONCELLI, RICCARDO; CIAMPI-GUILLARDI, MAISA; MARTINS, TULIO V.; BOUFLEUR, THAIS R.; MASSOLA JUNIOR, NELSON S.. First report of anthracnose caused by Colletotrichum truncatum on Digitaria insularis in Brazil. JOURNAL OF PLANT PATHOLOGY, v. N/A, p. 2-pg., 2023-07-17. (21/01606-6, 17/09178-8)