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Cell analysis evaluation of osseointegration in two different surfaces of titanium implants placed in bone defects filled or not with biomaterial

Grant number: 17/04017-6
Support type:Regular Research Grants
Duration: September 01, 2017 - August 31, 2019
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Valfrido Antonio Pereira Filho
Grantee:Valfrido Antonio Pereira Filho
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Assoc. researchers:Ana Paula Farnezi Bassi ; Guilherme dos Santos Trento ; Lucas Borin Moura ; Roberta Okamoto ; Rubens Spin Neto


The objective of this study is to evaluate the histological response of perimplant bone tissue contact with two different surfaces of dental implants placed in bone defects filled or not with biomaterial. Ninety-two titanium implants will be divided into four groups according to the type of surface treatment and the presence of biomaterial in the implant site: (G1) porous-hydrophilic surface without biomaterial, (G2) porous-hydrophilic surface with biomaterial, (G3) porous surface without biomaterial, and (G4) porous surface with biomaterial. The implants will be placed in the tibiae of forty-six rabbits and each animal will receive two implants. The samples will be evaluated by immunohistochemical analysis for detection of osteopontin, osteoprogesterin, receptor activator of nuclear factor kappa B ligand (RANK-L), osteocalcin, tatrate-resistant acid phosphatase, run-related transcription factor 2 antibodies and bone matrix proteins by immunoperoxidase detection and 3,3'diaminobenzidine. The analysis of bone quality will be performed by micro computed tomography. The bone-implant contact and bone area fraction occupied will be performed by histomorphometric evaluation. Finally, the gene expression of target genes related to bone formation such as Runx2, Alkaline Phosphatase (ALP), and Osteocalcin will be evaluated by Real Time Polymerase Chain Reaction (RT-PCR) in three samples of each group at the 60 day period. Data will be tabulated for statistical evaluation according to normality tests. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TRENTO, GUILHERME; DE A. CARVALHO, PEDRO HENRIQUE; DE C. REIS, ERIK N. R.; SPIN-NETO, RUBENS; BASSI, ANA PAULA F.; PEREIRA-FILHO, VALFRIDO ANTONIO. Bone formation around two titanium implant surfaces placed in bone defects with and without a bone substitute material: A histological, histomorphometric, and micro-computed tomography evaluation. CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH, v. 22, n. 2 FEB 2020. Web of Science Citations: 0.
TRENTO, GUILHERME DOS SANTOS; SPIN-NETO, RUBENS; FARNEZI BASSI, ANA PAULA; OKAMOTO, ROBERTA; CABRINI GABRIELLI, MARISA APARECIDA; PEREIRA-FILHO, VALFRIDO ANTONIO. Bone tissue formation around two titanium implant surfaces placed in bone defects filled with bone substitute material or blood clot: A pilot study. CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH, NOV 2019. Web of Science Citations: 0.

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