Sampling and processing of blood samples within the South American Youth/Child cARdiovascular and Environmental (SAYCARE) Study

• Grant number: 17/15594-4
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: September 01, 2017
• End date: February 28, 2018
• Field of knowledge: Health Sciences - Collective Health - Epidemiology
• Principal Investigator: Heraclito Barbosa de Carvalho
• Grantee: Heraclito Barbosa de Carvalho
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated research grant: 14/11468-6 - Design and implementation of the SAYCARE Study: South American Youth/Child Cardiovascular and Environmental Study, AP.R

Abstract

Objective: The prevalence of obesity and overweight in children and adolescents is increasing rapidly worldwide; however, scarce data have been reported from South America countries. With the purpose of assessing hyperlipidemia as well as insulin resistance and chronic inflammation, the evaluation of blood biomarkers such as glucose, lipids and lipoproteins as well as chronic inflammation proteins is required.Methods: In the context of the observational multicenter feasibility study, SAYCARE, held in children and adolescents (3 to 17 years) from seven South American cities, our aim was to assess the impact of pre analytical conditions on different blood biomarkers evaluated in 474 fresh samples of serum, in different country centers. Results: We also evaluated the stability of different biomarkers according to time and frozen storage within this feasibility study across the concordance of the results obtained from the 49 blood samples measured in two different centers.Conclusions: We observed excellent concordance observed, between Buenos Aires and Teresina vs São Paulo, samples could also be processed in each of these centers. This concordance is a consequence of the strict compliment of the pre analytical conditions previously established in the SAYCARE study. (AU)