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Composition and influence of the intestinal microbiome in mice, during the process of cachexia development, induced by transplantation of Lewis lung carcinoma (LLC) cells

Grant number: 17/08112-3
Support type:Regular Research Grants
Duration: December 01, 2017 - February 29, 2020
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Daniela Leite Jabes
Grantee:Daniela Leite Jabes
Home Institution: Pró-Reitoria Acadêmica. Universidade de Mogi das Cruzes (UMC). Campus da Sede Mogi das Cruzes. Mogi das Cruzes , SP, Brazil
Assoc. researchers:Luciana Campos Paulino ; Luiz Roberto Nunes ; Miguel Luiz Batista Junior

Abstract

A multitude of microorganisms inhabit the human body, outnumbering the host´s own cells by at least one order of magnitude. This complex micro-community, referred to as the microbiota, is composed by bacteria, archaea, viruses and eukaryotic microorganisms, most of them living in harmonic associations with their hosts. Nonetheless, there are situations in which imbalances verified in these relationships (called dysbioses) can contribute to the development of non-infectious pathologies (either local, or systemic). In this sense, current data indicate that changes in the composition of the intestinal microbiota appear to be particularly related to the development of metabolic syndromes, such as diabetes, obesity and, more recently, cachexia. Cachexia is recognized as a metabolic syndrome associated with several underlying diseases such as cancer, chronic kidney disease and chronic heart disease. Studies involving analysis of the bacterial microbiota from cachectic mice point to the existence of a correlation between the development of the syndrome and changes in the relative proportions of bacteria belonging to the genera Lactobacillus, Enterobacteriaceae and Parabacterioides. In addition, strategies aimed at restoring the original populations of these microorganisms seem to contribute to attenuate and/or reverse the cachectic condition. However, studies to verify the existence of correlations between the development of cachexia and population changes in other components of the gastrointestinal microbiota (notably fungi and viruses) have not yet been performed. In this sense, the project proposed herein aims to perform, for the first time, a comprehensive characterization of the changes that occur in the intestinal microbiota of mice from the C57BL/6 lineage (including bacteria, fungi and viruses), when these animals are induced to develop cachexia by transplantation of Lewis Lung Carcinoma (LLC) tumor cells. In addition, an unpublished functional inference analysis will be conducted using fecal microbiota before and after the establishment of cachexia. To achieve this goal, bioinformatics approaches will be performed to identify the metabolic-functional alterations that occur in the community of microorganisms, due to the changes of composition to which it is submitted. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARBOSA, DAVID ACIOLE; MENEGIDIO, FABIANO BEZERRA; ALENCAR, VALQUIRIA CAMPOS; GONCALVES, RAFAEL S.; SANTOS SILVA, JULIANA DE FATIMA; VILAS BOAS, RENATA OZELAMI; LIMA FAUSTINO DE MARIA, YARA NATERCIA; JABES, DANIELA LEITE; DE OLIVEIRA, REGINA COSTA; NUNES, LUIZ R. ParaDB: A manually curated database containing genomic annotation for the human pathogenic fungi Paracoccidioides spp.. PLoS Neglected Tropical Diseases, v. 13, n. 7 JUL 2019. Web of Science Citations: 0.
MENEGIDIO, FABIANO B.; BARBOSA, DAVID ACIOLE; GONCALVES, RAFAEL DOS S.; NISHIME, MARCIO M.; JABES, DANIELA L.; DE OLIVEIRA, REGINA COSTA; NUNES, LUIZ R. Bioportainer Workbench: a versatile and user-friendly system that integrates implementation, management, and use of bioinformatics resources in Docker environments. GIGASCIENCE, v. 8, n. 4 APR 2019. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.