Study of IGF-II growth factor expression and SF-1 and DAX-1 transcription factors in adrenocortical tumors in children and adults

• Grant number: 06/00244-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: July 01, 2006
• End date: August 31, 2008
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Ana Claudia Latronico Xavier
• Grantee: Madson Queiroz Almeida
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Adrenocortical tumorigenesis has been intensively investigated in last 10 yr. Loss of heterozygosity of the 11p15 locus leading to IGF-II gene over expression has been found in 93% of the malignant adrenocortical tumors and 8.6% of the benign tumors. However, the role of IGF-II expression in childhood adrenocortical tumorigenesis is yet to be determined. The transcription factors SF-1 and DAX-1, orphan members of the steroid nuclear receptor superfamily, which regulate the expression of adrenal hydroxylases, are essential for the development and differentiation of the adrenal cortex. SF-1 gene maps to 9q33.3, which is a region that has shown gain in childhood adrenocortical tumors. The aim of this study is to perform a quantitative analysis of IGF-II, SF-1 and DAX-1 gene expression and establish the prognostic value of these molecular markers in Brazilian adults and children with adrenocortical tumors. Fifty patients, adults or children of both sexes, with sporadic adrenocortical tumors will be studied. Representative samples of the adrenocortical tumors will be obtained during surgical procedure (adrenalectomy). Total RNA will be extracted from the tumor samples frozen in liquid nitrogen and cDNA synthesis will be carried out using reverse transcription. The IGF-II, SF-1 and DAX-1 gene expression will be determined by quantitative real-time PCR using a Taqman assay. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, MADSON Q.; BARISSON VILLARES FRAGOSO, MARIA CANDIDA; PACICCO LOTFI, CLAUDIMARA FERINI; SANTOS, MARIZA GERDULO; NISHI, MIRIAN YUMIE; SOARES COSTA, MARCIA HELENA; LERARIO, ANTONIO MARCONDES; MACIEL, CAROLINA CANTON; MATTOS, GABRIELE EBLING; LIMA JORGE, ALEXANDER AUGUSTO; et al. Expression of insulin-like growth factor-II and its receptor in pediatric and adult adrenocortical tumors. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, v. 93, n. 9, p. 3524-3531, SEP 2008. (06/00244-3)

ALMEIDA‚ M.Q.; BRITO‚ V.N.; LINS‚ T.S.S.; GUERRA-JUNIOR‚ G.; DE CASTRO‚ M.; ANTONINI‚ S.R.; ARNHOLD‚ I.J.P.; MENDONCA‚ B.B.; LATRONICO‚ A.C.. Long-term treatment of familial male-limited precocious puberty (testotoxicosis) with cyproterone acetate or ketoconazole. Clinical Endocrinology, v. 69, n. 1, p. 93-98, 2008. (05/04726-0, 06/00244-3)

DE SOUSA, G. R. V.; SOARES, I. C.; FARIA, A. M.; DOMINGUES, V. B.; WAKAMATSU, A.; LERARIO, A. M.; ALVES, V. A. F.; ZERBINI, M. C. N.; MENDONCA, B. B.; FRAGOSO, M. C. B. V.; et al. DAX1 Overexpression in Pediatric Adrenocortical Tumors: A Synergic Role with SF1 in Tumorigenesis. Hormone and Metabolic Research, v. 47, n. 9, p. 656-661, AUG 2015. (11/09092-0, 12/21272-6, 06/00244-3)

ALMEIDA, MADSON Q.; SOARES, IBERE CAUDURO; RIBEIRO, TAMAYA C.; FRAGOSO, MARIA CANDIDA B. V.; MARINS, LIDIANE V.; WAKAMATSU, ALDA; RESSIO, RODRIGO A.; NISHI, MIRIAN Y.; JORGE, ALEXANDER A. L.; LERARIO, ANTONIO M.; et al. Steroidogenic Factor 1 Overexpression and Gene Amplification Are More Frequent in Adrenocortical Tumors from Children than from Adults. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, v. 95, n. 3, p. 1458-1462, MAR 2010. (06/00244-3, 05/04726-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALMEIDA, Madson Queiroz. Expression of IGF-II, IGF-IR, SF-1 and DAX-1 genes in pediatric and adult adrenocortical tumors. 2008. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.