Th1 cellular response and its modulation in renal inflammatory injury secondary to ischemia and reperfusion

• Grant number: 06/04029-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2007
• End date: December 31, 2008
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Niels Olsen Saraiva Câmara
• Grantee: Vanessa Nunes de Paiva
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Renal ischemia/reperfusion injury (I/R)is the main cause of intrinsic ARF in the native and transplant kidneys. Recent data demonstrated that T CD4+ cells play an essential role as a mediator of kidneyaggression in I/R. However, the effector mechanisms involved are still poor understand, what complicates the drawing of strategies for limit the tissue injury. In this project, we hypothesize that the Th1 cytokines can be deleterious to the tissue kidney ischemic and that its production can indeed amplify the inflammatory cascade and inhibit the protective tissue response. In this way, we intend to analyze the functional and kidney morphological alterations in IL-12 deficient mice (as standard of deficient TH1) and IL-4 deficient mice(as standand of deficient TH2) subjected to renal I/R. Adoptive transfer of distinct cell populations for "empty" deficient mice and chromatin struture remodeling assays will also be tested. Cellular infiltration TH1 (CXCR3) and TH2 (CCR4) pattern will be studied by immunohistochemistry and the production of cytokines by macroarray. The role of the protective tissue response will be studied through the induction or chemical repression of the HO-1 before of the induction of injury of I/R. We expect to demonstrate that TH1 cytokines are the main effector mechanism by which T cells injury the tissue, and most importantly, that it can be modulate in pro of an anti-inflammatory scenario.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PAIVA, Vanessa Nunes de. Participation of Th1 and Th2 cytokines ischemia and reperfusion injury of kidney.. 2008. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.