Quantitative evaluation of Bacteroides fragilis enterotoxigenic strains and respective BFT gene subtypes in immunocompetent children with and without acute diarrhea

• Grant number: 07/03655-7
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: November 01, 2007
• End date: June 30, 2011
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Mario Júlio Ávila-Campos
• Grantee: Victor Rafael Castillo Merino
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Anaerobic gram-negative bacteria are predominant in indigenous microbiota of several mammals including human being. These bacteria are found in oral, intestinal and vaginal cavities. They are isolated from patients with different endogenous infections. Genus Bacteroides are formed by anaerobic gram-negative rods that constitute the intestinal resident microbiota in humans and animals. Bacteroides fragilis is an important microorganism because of production of different virulence factors such as capsules and proteolytic and hydrolitic enzymes. Some B. fragilis strains are able to produce a metalloprotease toxin called fragilisyn which is related to diarrhea processes and they are called enterotoxigenic Bacteroides fragilis (ETBF). Moreover, these anaerobic bacteria are involved in several infection processes and they are associated to morbidity and mortality of patients. In Brazil, the identification and detection of anaerobic bacteria in clinic laboratories is not a routine. The aim of this study is to quantitatively evaluate the presence of ETBF from immunocompetent children with and without acute diarrhea, as well as the presence of respective subtypes of the bft gene by using RT-PCR method. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MERINO, V. R. C.; NAKANO, V.; LIU, C.; SONG, Y.; FINEGOLD, S. M.; AVILA-CAMPOS, M. J.. Quantitative Detection of Enterotoxigenic Bacteroides fragilis Subtypes Isolated from Children with and without Diarrhea. Journal of Clinical Microbiology, v. 49, n. 1, p. 416-418, JAN 2011. (07/03655-7)

MERINO, VICTOR R. C.; NAKANO, VIVIANE; FINEGOLD, SYDNEY M.; AVILA-CAMPOS, MARIO J.. Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea. SCIENTIFICA, v. 2014, p. 4-pg., 2014-01-01. (07/03655-7, 09/03792-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MERINO, Victor Rafael Castillo. Quantitative analysis of enteropathogens in children with and without diarrhea in São Paulo city, SP.. 2012. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.