Effects of equine inflammation on hepcidin expression and plasma iron concentration

• Grant number: 07/05008-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2007
• End date: December 31, 2010
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Alexandre Secorun Borges
• Grantee: José Paes de Oliveira Filho
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Equine serum or plasma iron concentration drops quickly during inflammation, this test is a reliable procedure for systemic inflammatory process diagnosis. Accumulation of iron inside macrophages and reduction of the intestinal absorption of this element causes hypoferremia during systemic inflammatory process. These mechanisms are mediated by the hepcidin, a 25 amino acids peptide synthesized mainly by the hepatocytes in response to iron stores and inflammation. The higher hepcidin expression during inflammation restricts the iron availability, necessary for pathogenic microorganism growth. This increase in hepcidin expression can be particularly useful during initial periods of inflammation, before adaptive immunity occurs. The hepcidin sequencing has already been performed in human, rat, mouse, swine, fish and dog; however hepcidin in equines has not yet been sequenced. Here we propose to sequence the responsible gene for hepcidin codification in equine, to verify its expression in different tissues and to evaluate if inflammatory induction decreases plasmatic iron concentration and check if this was preceded by an increase in hepcidin expression. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA-FILHO, JOSE P.; BADIAL, PERES R.; CUNHA, PAULO HENRIQUE J.; BORDON, ANA PAULA; ARAUJO, JR., JOAO P.; DIVERS, THOMAS J.; WINAND, NENA J.; BORGES, ALEXANDRE S.. Inflamação induzida por Adjuvante de Freund: achados clínicos e efeitos sobre a expressão do RNAm da hepcidina em equinos. Pesquisa Veterinária Brasileira, v. 34, n. 1, p. 51-56, JAN 2014. (07/05008-9, 07/07344-6)

OLIVEIRA FILHO, JOSE P.; BADIAL, PERES R.; CUNHA, PAULO H. J.; CRUZ, TAIS F.; ARAUJO, JR., JOAO P.; DIVERS, THOMAS J.; WINAND, NENA J.; BORGES, ALEXANDRE S.. Cloning, sequencing and expression analysis of the equine hepcidin gene by real-time PCR. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, v. 135, n. 1-2, p. 34-42, MAY 15 2010. (07/07344-6, 07/05008-9)

OLIVEIRA-FILHO, JOSE P.; BADIAL, PERES R.; CUNHA, PAULO H. J.; PEIRO, JULIANA R.; ARAUJO, JR., JOAO P.; DIVERS, THOMAS J.; WINAND, NENA J.; BORGES, ALEXANDRE S.. Lipopolysaccharide infusion up-regulates hepcidin mRNA expression in equine liver. Innate Immunity, v. 18, n. 3, p. 438-446, JUN 2012. (07/07344-6, 07/05008-9)

OLIVEIRA-FILHO, JOSE P.; BADIAL, PERES R.; CUNHA, PAULO HENRIQUE J.; BORDON, ANA PAULA; ARAUJO, JOAO P., JR.; DIVERS, THOMAS J.; WINAND, NENA J.; BORGES, ALEXANDRE S.. Freund's adjuvant-induced inflammation: clinical findings and its effect on hepcidin mRNA expression in horses. Pesquisa Veterinária Brasileira, v. 34, n. 1, p. 6-pg., 2014-01-01. (07/05008-9, 07/07344-6)