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Investigation of hyperfine interactions in DNA and antibody of different lineages of mice infected by T. cruzi by Perturbed gamma-gamma angular correlation spectroscopy

Grant number: 08/00844-6
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2008
Effective date (End): October 31, 2011
Field of knowledge:Engineering - Nuclear Engineering - Applications of Radioisotopes
Principal researcher:Artur Wilson Carbonari
Grantee:Andréia dos Santos Silva
Home Institution: Instituto de Pesquisas Energéticas e Nucleares (IPEN). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:03/10156-6 - The IEA-R1 nuclear reactor as a source of neutrons and its use in research, development and training of human resources, AP.PRNX.TEM


The knowledge of the behavior of biomolecules is fundamental to understand phenomena like metabolic dynamic of biologic systems, immunologic processes, therapeutic processes, etc.. Although many investigations have been carried out in order to study such phenomena, the best interpretations are from a macroscopic view (cellular level). However, to better know the biologic system it is necessary to understand the local interactions in biomolecules (molecular level). One of the most promising way to do such investigation is through the measurement of hyperfine interactions (HFI) in a particular atom-site of a biomolecule. Hyperfine interaction can provide information about the neighborhood of a certain probe atom in a nanometric scale via interactions between the moments of the nucleus of this probe atom and spins and charges around it. Nuclear Magnetic Resonance (NMR) is one of the HFI techniques that can be used. However, NMR is not capable to observe local dynamics in a biomolecule. Perturbed angular correlation (PAC) is another technique that provide with high accuracy static as well as dynamic hyperfine parameters related to phenomena that occur in compounds or in biomolecules. In this project it is intended, in a first step, to develop the utilization of PAC spectroscopy to study DNA, including the sample preparation and data analysis. In a second part, the PAC spectroscopy will be used to investigate phenomena in antibody molecules and DNA of different mouse lineages when infected by Y clone of T. cruzi

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