Molecular caracterization of rapidly growing micobacteria associated or not to outbreaks in laparoscopic surgeries for the development of a quick identification test

• Grant number: 08/01451-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2008
• End date: February 29, 2012
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Sylvia Luisa Pincherle Cardoso Leão
• Grantee: Cristianne Kayoko Matsumoto
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 06/01533-9 - Mycobacteria of medical significance in Brazil: molecular characterization, interaction with the environment and with macrophages, AP.TEM

Abstract

Outbreaks of infections by non tuberculous rapidly growing mycobacteria in laparoscopic surgeries are occurring in different States since 2004 (Pará, Goiás, Rio de Janeiro, Espírito Santo, Rio Grande do Sul and Paraná). Isolates were identified by the molecular method known as PCR-Restriction Enzyme Analysis (PRA-hsp65) and presented a profile common to three micobacterial species - Mycobacterium abscessus, Mycobacterium massiliense and Mycobacterium bolletii. The strain responsible for the outbreaks was later on identified by DNA sequencing as M. massiliense. Preliminary analyses with isolates from Pará, Rio de Janeiro, Goiás and Espírito Santo, using the molecular typing method Pulsed Field Gel Electrophoresis (PFGE), showed that patients were infected by a unique strain of M. massiliense. This strain can only be identified at this moment by DNA sequencing and PFGE, both expensive and not generally available methods. In this project 58 isolates from the outbreak in Espírito Santo, and 53 strains presenting the same PRA-hsp65 pattern, but not related to these outbreaks, isolated in the same period in different areas of the country will be studied. The genetic diversity of these strains will be evaluated by sequencing DNA regions conserved in genomes of mycobacteria. We intend to develop a quick molecular test for identification of the outbreak strain, capable of differentiating it from non-related strains. To achieve this, obtained DNA sequences will be compared for identification of genetic signatures that could differentiate outbreak from non-outbreak strains. The developed molecular test will then be implemented in Lacens from different States for detection of new cases and epidemiological surveillance. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATSUMOTO, CRISTIANNE KAYOKO; CHIMARA, ERICA; RAMOS, JESUS PAIS; DIAS CAMPOS, CARLOS EDUARDO; DE SOUZA CALDAS, PAULO CESAR; BATISTA LIMA, KARLA VALERIA; LOPES, MARIA LUIZA; DUARTE, RAFAEL SILVA; LEAO, SYLVIA CARDOSO. Rapid tests for the detection of the Mycobacterium abscessus subsp bolletii strain responsible for an epidemic of surgical-site infections in Brazil. Memórias do Instituto Oswaldo Cruz, v. 107, n. 8, p. 969+, DEC 2012. (08/01451-8)

CRISTIANNE KAYOKO MATSUMOTO; ERICA CHIMARA; JESUS PAIS RAMOS; CARLOS EDUARDO DIAS CAMPOS; PAULO CESAR DE SOUZA CALDAS; KARLA VALERIA BATISTA LIMA; MARIA LUIZA LOPES; RAFAEL SILVA DUARTE; SYLVIA CARDOSO LEÃO. Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil. Memórias do Instituto Oswaldo Cruz, v. 107, n. 8, p. 969-977, 2012-12-00. (08/01451-8)

MATSUMOTO, CRISTIANNE KAYOKO; CHIMARA, ERICA; BOMBARDA, SIDNEY; DUARTE, RAFAEL SILVA; LEAO, SYLVIA CARDOSO. Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates. Journal of Clinical Microbiology, v. 49, n. 1, p. 62-68, JAN 2011. (08/01451-8, 06/01533-9)