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Analysis of different carriers for BMP2 in the bone repair process in surgically created cavities. A histomorphometric, immunohistochemical and fluorochrome study in rats.

Grant number: 08/10516-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2009
Effective date (End): May 31, 2011
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Roberta Okamoto
Grantee:Eloá Rodrigues Luvizuto
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Extensive bone defects in maxillofacial region can be corrected with autogenous grafts; otherwise, the disadvantages of the therapeutics modality take the research for new bone substitutes. Considering this, the use of osteoinductive factors, as bone morphogenetic proteins (BMPs) have been an alternative. Otherwise, carriers and scaffolds are essential to maintain the osteoinduction capacity of BMPs. Therefore, the present project has the aim to evaluate the biological role of different BMP2 carriers (demineralized bone matrix gel, calcium phosphate cement and polylactic and polyglicolic copolymer) in the process of bone repair in surgically created cavities in rats calvaria. Two critical size defects (A and B), with 5 mm diameter, will be prepared with trefine and slow rotation "motor" over intense irrigation for each animal. The animals will be divided in 4 groups; in Group I, the A defect will be filled with blood clot and the B defect will be filled with particulated autogenous bone; in Group II, the A defect will be filled with demineralized bone matrix gel plus BMP2 and the B defect will be filled with demineralized bone matrix gel; in Group III, the A defect will be filled with calcium phosphate cement plus BMP2 and The B defect will be filled with the calcium phosphate cement; in Group IV, the A defect will be filled with polylactic and polyglicolic acid copolymers plus BMP2 and the B defect will be filled with polylactic and polyglicolic acid copolymers. The animals will be sacrificed at 5, 15 and 30 pos operative days. The analysis of biologic mechanisms involved in the bone repair process will be obtained by histomorphometric use of fluorochromes and immunohistochemistry through the expression of Runx2, alkaline phosphatase, osteopontin, osteocalcin, TRAP and Type I collagen proteins, that are involved in osteoinductive, mineralization and bone resorption process.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA DE OLIVEIRA, JULIO CESAR; LUVIZUTO, ELOA RODRIGUES; SONODA, CELSO KOOGI; OKAMOTO, ROBERTA; GARCIA-JUNIOR, IDELMO RANGEL. Immunohistochemistry evaluation of BMP-2 with beta-tricalcium phosphate matrix, polylactic and polyglycolic acid gel, and calcium phosphate cement in rats. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, v. 21, n. 2, p. 247-258, . (08/10516-6)
LUVIZUTO, ELOA R.; TANGL, STEFAN; ZANONI, GERALD; OKAMOTO, TETUO; SONODA, CELSO K.; GRUBER, REINHARD; OKAMOTO, ROBERTA. The effect of BMP-2 on the osteoconductive properties of beta-tricalcium phosphate in rat calvaria defects. Biomaterials, v. 32, n. 15, p. 3855-3861, . (08/10516-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LUVIZUTO, Eloá Rodrigues. O efeito da BMP-2 sobre as propriedades osteocondutoras do beta-tricálcio fosfato em defeitos de calvária de ratos. 2011. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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